Title Mesenchymal stem cell assays and applications / edited by Mohan C. Vemuri, Lucas G. Chase, Mahendra S. Rao

Published New York : Humana Press, ©2011

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Description 1 online resource (xvi, 534 pages) Series Methods in molecular biology, methods and protocols ; v. 698 Methods in molecular biology (Clifton, N.J.) ; v. 698 Contents Mesenchymal stem cell assays and applications / Mohan C. Vemuri, Lucas G. Chase, and Mahendra S. Rao -- Isolation and expansion of mesenchymal stem cells/multipotential stromal cells from human bone marrow / Patrice Penfornis and Radhika Pochampally -- Standardized isolation of human mesenchymal stromal cells with red blood cell lysis / Patrick Horn, Simone Bork, and Wolfgang Wagner -- Isolation and growth of adipose tissue-derived stem cells / Vladimir Zachar, Jeppe Groundahl Rasmussen, and Trine Fink -- Isolation, culture, and characterization of human umbilical cord stroma-derived mesenchymal stem cells / Alp Can and Deniz Balci -- Isolation and culture of human cord blood-derived mesenchymal stem cells under low oxygen conditions / Anita Laitinen, Johanna Nystedt, and Saara Laitinen -- Amniotic and placental mesenchymal stem cell isolation and culture / Justin D. Klein and Dario O. Fauza -- Manufacture of clinical grade human placenta-derived multipotent mesenchymal stromal cells / Nina Ilic [and others] -- Method to isolate and culture expand human dental pulp stem cells / Stan Gronthos [and others] -- Isolation and culture of human multipotent stromal cells from the pancreas / Karen L. Seeberger, Alana Eshpeter, and Gregory S. Korbutt -- Derivation and characterization of human ESC-derived mesenchymal stem cells / Ruenn Chai Lai, Andre Choo, and Sai Kiang Lim -- Isolation and culture of rodent bone marrow-derived multipotent mesenchymal stromal cells / Nance Beyer Nardi and Melissa Camassola -- Cryopreservation and revival of mesenchymal stromal cells / Mandana Haack-Sorensen and Jens Kastrup -- Dynamic expansion culture for mesenchymal stem cells / Hicham Majd [and others] -- Ex vivo expansion of human mesenchymal stem cells on microcarriers / Francisco dos Santos [and others] -- Osteogenic differentiation of human multipotent mesenchymal stromal cells / Deepak M. Gupta, Nicholas J. Panetta, and Michael T. Longaker -- Assays of osteogenic differentiation by cultured human mesenchymal stem cells / Ulf Krause, Anja Seckinger, and Carl A. Gregory -- Bioreactor cultivation of functional bone grafts / Warren L. Grayson [and others] -- Adipogenic differentiation of human mesenchymal stem cells / Trine Fink and Vladimir Zachar -- Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells : tips and tricks / Luis A. Solchaga, Kitsie J. Penick, and Jean F. Welter -- Use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering / Zhaodi Gong and Laura E. Niklason -- Dopaminergic neuronal differentiation protocol for human mesenchymal stem cells / Katarzyna A. Trzaska and Pranela Rameshwar -- Hepatic differentiation of mesenchymal stem cells : in vitro strategies / Sarah Snykers [and others] -- Hepatic transplantation of mesenchymal stem cells in rodent animal models / Bruno Christ, Sandra Bruckner, and Peggy Stock -- Phenotypic analysis and differentiation of murine mesenchymal stem cells / Lindolfo da Silva Meirelles and Dimas Tadeu Covas -- Immunohistochemical analysis of human mesenchymal stem cells differentiating into chondrogenic, osteogenic, and adipogenic lineages / Zheng Yang, Jacqueline Frida Schmitt, and Eng Hin Lee -- Panel development for multicolor flow-cytometry testing of proliferation and immunophenotype in hMSCs / Jolene A. Bradford and Scott T. Clarke -- Simplified PCR assay for detecting early stages of multipotent mesenchymal stromal cell differentiation / Shayne E. Boucher -- Transcriptome analysis of common gene expression in human mesenchymal stem cells derived from four different origins / Tzu-Hao Wang, Yun-Shien Lee, and Shiaw-Min Hwang -- Comparison of microarray and quantitative real-time PCR methods for measuring microRNA levels in MSC cultures / Cynthia Camarillo, Mavis Swerdel, and Ronald P. Hart -- Two dimensional gel electrophoresis analysis of mesenchymal stem cells / Monique Provansal [and others] -- Proteomic analysis of human mesenchymal stem cells / Guo Li [and others] -- Metabolic labeling and click chemistry detection of glycoprotein markers of mesenchymal stem cell differentiation / Courtenay Hart [and others] -- BacMam-mediated gene delivery into multipotent mesenchymal stromal cells / Michael O'Grady [and others] -- Cell surface engineering of mesenchymal stem cells / Debanjan Sarkar [and others] Summary Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells Analysis celbiologie cellular biology stamcellen stem cells Cellular Biology Celbiologie Bibliography Includes bibliographical references and index Notes English Print version record Subject Mesenchymal stem cells. Cells. Cultures (Biology) Medical laboratory technology. Connective tissue cells. Anatomy. Multipotent stem cells. Cell culture. Cytology -- Technique. Stem cells. Cells Culture Techniques Clinical Laboratory Techniques Connective Tissue Cells Investigative Techniques Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Mesenchymal Stem Cells Multipotent Stem Cells Stromal Cells Cells, Cultured Cell Culture Techniques Cytological Techniques Stem Cells anatomy. Stem cells Multipotent stem cells Medical laboratory technology Cytology -- Technique Cultures (Biology) Connective tissue cells Cells Cell culture Anatomy Mesenchymal stem cells Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Vemuri, Mohan C. Chase, Lucas G. Rao, Mahendra S. ISBN 9781607619994 1607619997

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