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Title Mesenchymal stem cells : methods and protocols / edited by Massimiliano Gnecchi
Edition Second edition
Published New York : Humana Press : Springer, [2016]


Description 1 online resource (xix, 570 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1416
Methods in molecular biology (Clifton, N.J.) ; v. 1416. 1064-3745
Contents Mesenchymal stromal cells in hematopoietic stem cell transplantation / Maria Ester Bernardo and Franco Locatelli -- Bone tissue engineering : past-present-future / Rodolfo Quarto and Paolo Giannoni -- Mesenchymal stem cells for osteochondral tissue engineering / Johnathan Ng, Jonathan Bernhard, and Gordana Vunjak-Novakovic -- Mesenchymal stem cells in cardiology / Ian A. White [and others] -- Mesenchymal stem cells in kidney repair / Marina Morigi, Cinzia Rota, and Giuseppe Remuzzi -- Mesenchymal stem cells in lipogems, a reverse story : from clinical practice to basic science / Carlo Tremolada [and others] -- Paracrine mechanisms of mesenchymal stem cells in tissue repair / Massimiliano Gnecchi [and others] -- Protocols for in vitro differentiation of human mesenchymal stem cells into osteogenic, chondrogenic and adipogenic lineages / Maria Chiara Ciuffreda [and others] -- Colony forming unit assays / Patrice Penfornis and Radhika Pochampally -- Methods and strategies for lineage tracing of mesenchymal progenitor cells / R. Wilder Scott and T. Michael Underhill -- Isolation of mouse bone marrow mesenchymal stem cells / Siddaraju V. Boregowda, Veena Krishnappa, and Donald G. Phinney -- Isolation of pig bone marrow-derived mesenchymal stem cells / Dries A.M. Feyen [and others] -- Isolation, culture, and phenotypic characterization of mesenchymal stromal cells from the amniotic membrane of the human term placenta / Marta Magatti [and others] -- Isolation, culture, and characterization of human umbilical cord blood-derived mesenchymal stromal cells / Karen Bieback and Philipp Netsch -- Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens / Luigi Balducci and Giulio Alessandri -- Optimization of mesenchymal stem cells to increase their therapeutic potential / Minh Quan Vu [and others] -- Directed differentiation of human-induced pluripotent stem cells to mesenchymal stem cells / Qizhou Lian [and others] -- Isolation and manufacture of clinical-grade bone marrow-derived human mesenchymal stromal cells / Renuka P. Miller and Patrick J. Hanley -- Quality control assays for clinical-grade human mesenchymal stromal cells : methods for ATMP release / Marina Radrizzani [and others] -- Quality control assays for clinical-grade human mesenchymal stromal cells : validation strategy / Marina Radrizzani [and others] -- Cryopreservation and revival of human mesenchymal stromal cells / Mandana Haack-Sørensen, Annette Ekblond, and Jens Kastrup -- Clinical-grade manufacturing of therapeutic human mesenchymal stem/ctromal cells in microcarrier-based culture systems / Ana Fernandes-Platzgummer [and others] -- GMP-compliant expansion of clinical-grade human mesenchymal stromal/stem cells using a closed hollow fiber bioreactor / Christina Barckhausen [and others] -- Engineering small-scale and scaffold-based bone organs via endochondral ossification using adult progenitor cells / Celeste Scotti [and others] -- Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture / Thasaneeya Kuboki and Satoru Kidoaki -- Testing the paracrine properties of human mesenchymal stem cells using conditioned medium / Patrizia Danieli [and others] -- Tips on how to collect and administer the mesenchymal stem cell secretome for central nervous system applications / F.G. Teixeira, S.C. Serra, and A.J. Salgado -- Soluble factors from human fetal bone marrow-derived mesenchymal stem cells : preparation of conditioned medium and its effect on tumor cells / Jerry K.Y. Chan and Paula Lam -- Isolation and characterization of exosome from human embryonic stem cell-derived C-Myc-immortalized mesenchymal stem cells / Ruenn Chai Lai [and others] -- Transcriptomic analysis of adult renal derived mesenchymal stem-like cells / Jose Gomez, Jeffrey Schmeckpeper, and Maria Mirotsou -- Proteomic analysis of mesenchymal stem cells / Vitor Marcel Faça [and others] -- Unraveling mesenchymal stem cells' dynamic secretome through nontargeted proteomics profiling / Sandra I. Anjo [and others] -- Identification of factors produced and secreted by mesenchymal stromal cells with the SILAC method / Beatriz Rocha [and others]
Summary Annotation Mesenchymal stem cells (MSC) represent one of the most interesting progenitors to date, due to their biodiverse functionalities. Among the fascinating multiple properties of MSC are their supportive roles in wound healing and in the regeneration of damaged tissues and organs. Although MSC research is progressively bridging to more consolidated clinical applications, it still represents a dynamically developing field, where a variety of intriguing aspects remain to be addressed. This volume represents a comprehensive summary gathering a panel of up-to-date articles which combine the diverse MSC biological functionalities and their potential in translational cell therapy, as highlighted from different angles with a broad interdisciplinary perspective
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF of title page (Springer Protocols, viewed May 31, 2016)
Subject Mesenchymal stem cells -- Laboratory manuals
Multipotent stem cells -- Cytology -- Laboratory manuals
Cell culture.
Cytology -- Technique.
Mesenchymal Stem Cells -- cytology
Cell Culture Techniques
Cells, Cultured
Cytological Techniques
Multipotent Stem Cells -- cytology
Stromal Cells -- cytology
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Cytology -- Technique
Cell culture
Mesenchymal stem cells
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Gnecchi, Massimiliano, editor.
LC no. 2016936658
ISBN 9781493935840