| Description |
1 online resource (xxxviii, 477 pages, 22 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology, 0076-6879 ; v. 420 |
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Methods in enzymology ; v. 420. 0076-6879
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| Contents |
In vitro experimentation and research tools: Human embryo culture / A. Mercader ... [et al.] -- Characterization and evaluation of human embryonic stem cells / C. Xu ... [et al.] -- Feeder-free culture of human embryonic stem cells / M. Amit ... [et al.] -- Transgene expression and RNA interference in embryonic stem cells / H. Zaehres ... [et al.] -- Lentiviral vector-mediated gene delivery into human embryonic stem cells / M. Gropp ... [et al.] -- The use of retroviral vectors for gene transfer into hematopoietic stem cells / J. Holst ... [et al.] -- Engineering embryonic stem cells with recombinase systems / F. Schnutgen ... [et al.] -- Gene trapping in embryonic stem cells / W.L. Stanford ... [et al.] -- Genechips in stem cell research / J. Hipp ... [et al.] -- Microarray analysis of stem cells and differentiation / H.Y. Chang ... [et al.] -- Purification of hematopoietic stem cells using the side population / K.K. Lin ... [et al.] -- Cellular reprogramming / S. Agarwal ... [et al.] -- Tissue engineering and regenerative medicine: Tissue engineering using adult stem cells / D. Eberli ... [et al.] -- Tissue engineering using human embryonic stem cells / S. Cohen ... [et al.] -- Engineering cardiac tissue from embryonic stem cells / X. Guo ... [et al.] -- Mesenchymal stem cells and tissue engineering / N.W. Marion ... [et al.] -- Bone reconstruction with bone marrow stromal cells / W. Liu ... [et al.] -- Engineering three-dimensional tissue structures using stem cells / J. Zoldan ... [et al.] -- Immunogenicity of embryonic stem cells and their progeny / M. Drukker ... [et al.] -- Manufacturing considerations for clinical uses of therapies derived from stem cells / D.J. Weber |
| Summary |
This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conven |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Stem cells -- Research -- Handbooks, manuals, etc.
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Stem cells.
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Tissue engineering.
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Stem Cells.
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Tissue Engineering.
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| Genre/Form |
Handbooks and manuals.
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| Form |
Electronic book
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| Author |
Klimanskaya, Irina.
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|
Lanza, R. P. (Robert Paul), 1956-
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| ISBN |
0080469647 (electronic bk.) |
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012373651X (electronic bk.) |
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9780080469645 (electronic bk.) |
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9780123736512 (electronic bk.) |
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