The theory and practice of in situ hybridization-- Oligonucleotide probes for in situ hybridization-- Detection of mRNA in tissue sections with radiolabelled riboprobes-- In situ hybridization of mRNA with hapten labelled probes-- Two colour in situ hybridization-- Electron microscope in situ hybridization: the non-isotopic post-embedding procedure-- Detection of genomic sequences by fluorescence in situ hybridization to chromosomes-- Gene expression databases
Summary
In situ hybridization is used to reveal the location of specific nucleic acid sequences in tissues or on chromosomes and as such is an essential technique for both fundamental and clinical research. In Situ Hybridization: A Practical Approach 2/e is a thoroughly updated new edition of one of the most successful Practical Approach books. It contains protocols for all the major techniques of in situ hybridization, written by authors with extensivepractical experience. The procedures described are applicable to a wide variety of systems and will be useful to all who wish to carry out in situ hybr
