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Title Herpes simplex virus protocols / edited by S. Moira Brown and Alasdair R. MacLean
Published Totowa, N.J. : Humana Press, ©1998


Description 1 online resource (xi, 419 pages) : illustrations
Series Methods in molecular medicine ; 10
Methods in molecular medicine ; 10.
Contents HSV growth, preparation, and assay / J. Harland and S.M. Brown-HSV entry and spread / C.A. Maclean-Preparation of HSV-DNA and production of infectious virus / A.R. Maclean-HSV mutagenesis / R.S. Coffin-Saturating mutagenesis and characterization of a herpesvirus genome using in vivo reconstitution of virus from cloned subgenomic regions / N. de Wind, M. van Zijl and A. Berns-Construction and use of cell lines expressing HSV genes / C. Entwisle-Analysis of HSV polypeptides / L. Haarr and N. Langeland-Protein purification / J. Conner-Expression and purification of secreted forms of HSV glycoproteins from baculovirus-infected insect cells / S.H. Willis [and others]-Crystallization of macromolecules for three-dimensional structure determination / B. Luisi, M. Anderson and G. Hope-Direct immunogold labeling of herpesvirus suspensions / L.M. Stannard-Expression of HSV proteins in bacteria / E.A. McKie-In vitro systems to analyze HSV transcript processing / A. Phelan and J.B. Clements-Analysis of HSV-1 transcripts by RNA-pcr / J.G. Spivack-Transient assays for HSV origin and replication protein function / N.D. Stow-HSV amplicons in gene therapy / N. Frenkel and R. Sarid-Analyses of HSV proteins for posttranslational modifications and enzyme functions / J.A. Blaho and B. Roizman-HSV-cellular protein interactions / D.S. Latchman-Models of recurrent infection with HSV in the skin and eye of the mouse / T.J. Hill and C. Shimeld-Pathogenesis and molecular biology of HSV latency and ocular reactivation in the rabbit / J.M. Hill, R. Wen and W.P. Halford-HSV latency in vitro : In situ hybridization methods / C.L. Wilcox and R.L. Smith-Assessing cell-mediated immune responses to HSV in murine systems / T.A. Banks, M.J. Hariharan and B.T. Rouse-Assays for HSV gene expression during establishment and maintenance of latent infection / P. Speck and S. Efstathiou-Analysis of HSV-DNA and RNA using the polymerase chain reaction / R. Ramakrishnan, D.J. Fink and M. Levine-HSV vectors for gene therapy / D.C. Bloom-Investigation of the anti-HSV activity of candidate antiviral agents / D.J. Dargan
Summary An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Herpes simplex virus -- Laboratory manuals
Herpes Simplex -- diagnosis
Simplexvirus -- physiology
Herpes simplex virus
Herpes simplex virus.
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Brown, S. Moira.
MacLean, Alasdair R.
LC no. 97035999
ISBN 9781592595945