| Description |
1 online resource (xi, 349 pages) : illustrations |
| Series |
Methods in molecular medicine ; 73 |
|
Methods in molecular medicine ; 73. 1543-1894
|
| Contents |
The medical significance of shiga toxin-producing escherichia coli infections. An overview / M.A. Karmali -- Methods for detection of stec in humans. An overview / J.C. Paton and A.W. Paton -- Serological methods for the detection of stec infections / M. Bitzan and H. Karch -- Detection and characterization of stec in stool samples using pcr / A.W. Paton and J.C. Paton -- Molecular typing methods for stec / H. Watanabe [and others] -- Stec in the food chain. Methods for detection of stec in food samples / M. Bulte -- Stec as a veterinary problem. Diagnostics and prophylaxis in animals / L.H. Wieler and R. Bauerfeind -- Cellular microbiology of stec infections. An overview / F. Ebel and D. Philpott -- Analysis of pathogenicity islands of stec / T.A. Oelschlaeger [and others] -- Generation of isogenic deletion mutants of stec / S. Djafari, N.D. Hauf and J.F. Tyczka -- Generation of monoclonal antibodies against secreted proteins of stec / K. Niebuhr and F. Ebel -- Microscopic methods to study stec. Analysis of the attaching and effacing process / S. Knutton -- Detection and characterization of ehec-hemolysin / H. Schmidt and R. Benz -- Shiga toxin receptor glycolipid binding. Pathology and utility / C.A. Lingwood -- Methods for the purification of shiga toxin 1 / A. Nutikka, B. Binnington-Boyd and C.A. Lingwood -- Methods for the identification of host receptors for shiga toxin / A. Nutikka, B. Binnington-Boyd and C.A. Lingwood -- Shiga toxin b-subunit as a tool to study retrograde transport / F. Mallard and L. Johannes -- Measuring ph within the golgi complex and endoplasmic reticulum using shiga toxin / J.H. Kim -- Detection of shiga toxin-mediated programmed cell death and delineation of death-signaling pathways / N.L. Jones -- Interaction of shiga toxin with endothelial cells / M. Bitzan and D.M. te Loo -- Shiga toxin interactions with the intestinal epithelium / C.M. Thorpe, B.P. Hurley and D.W. Acheson -- Protocols to study effects of shiga toxin on mononuclear leukocytes / C. Menge -- Animal models for stec-mediated disease / A.R. Melton-Celsa and A.D. O'Brien -- Gnotobiotic piglets as an animal model for oral infection with o157 and non-o157 serotypes of stec / F. Gunzer [and others] -- Bovine escherichia coli o157:H7 infection model / E.A. Dean-Nystrom |
| Summary |
Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods. Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
|
English |
|
Print version record |
|
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Escherichia coli infections -- Laboratory manuals
|
|
Verocytotoxins -- Laboratory manuals
|
|
Escherichia coli Infections -- pathology
|
|
Shiga Toxin -- analysis
|
|
MEDICAL -- Infectious Diseases.
|
|
Escherichia coli infections
|
|
Verocytotoxins
|
| Genre/Form |
Handbooks and manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Philpott, Dana.
|
|
Ebel, Frank.
|
| LC no. |
2002068800 |
| ISBN |
9781592593163 |
|
159259316X |
|
0896039390 |
|
9780896039391 |
|
1280843160 |
|
9781280843167 |
|
9786610843169 |
|
6610843163 |
|
