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Book Cover

Title Natural products from marine algae : methods and protocols / edited by Dagmar B. Stenfel, Solene Connan
Published New York, NY : Humana Press : Springer, [2015]


Description 1 online resource (xiv, 439 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1308
Methods in molecular biology (Clifton, N.J.) ; v. 1308. 1064-3745
Contents Marine algae: A source of biomass for biotechnological applications -- Structure and function of macroalgal natural products -- Spectrophotometric assays of major compounds extracted from algae -- Extraction and enrichment of protein from red and green macroalgae -- Extraction and purification of r-phycoerythrin from marine red algae -- Extraction and analysis of mycosporine-like amino acids in marine algae -- Extraction and purification of phlorotannins from brown algae -- Enzyme-enhanced extraction of antioxidant ingredients from algae -- Microwave-assisted extraction of fucoidan from marine algae -- Extraction and analysis of oxylipins from macroalgae illustrated on the example gracilaria vermiculophylla -- Lipids and fatty acids in algae: Extraction, fractionation into lipid classes, and analysis by gas chromatography coupled with flame ionization detector (gc-fid) -- Hrmas nmr analysis of algae and identification of molecules of interest via conventional 1d and 2d nmr: Sample preparation and optimization of experimental conditions -- Extraction, purification, and nmr analysis of terpenes from brown algae -- Extraction, isolation, and identification of sesquiterpenes from laurencia species -- The use of hplc for the characterization of phytoplankton pigments -- Characterization of phlorotannins from brown algae by lc-hrms -- Analysis of betaines from marine algae using lc-ms-ms -- Analysis of marine biotoxins using lc-ms/ms -- Fucoidan analysis by tandem maldi-tof and esi mass spectrometry -- Determination of substitution patterns of galactans from green seaweeds of the bryopsidales -- Structural characterization of a hybrid carrageenan-like sulfated galactan from a marine red alga furcellaria lumbricalis -- Characterization of alginates by nuclear magnetic resonance (nmr) and vibrational spectroscopy (ir, nir, raman) in combination with chemometrics -- Imaging and identification of marine algal bioactive compounds by surface enhanced raman spectroscopy (sers) -- In vitro protocols for measuring the antioxidant capacity of algal extracts -- Disk diffusion assay to assess the antimicrobial activity of marine algal extracts -- Screening of a marine algal extract for antifungal activities -- Protocol for assessing antifouling activities of macroalgal extracts
Summary Annotation This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed June 29, 2015)
Subject Marine natural products -- Laboratory manuals
Marine algae -- Laboratory manuals
Marine algae.
Botany & plant sciences.
Science -- Life Sciences -- Biochemistry.
Science -- Life Sciences -- Botany.
Marine algae
Marine natural products
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Stengel, Dagmar B., editor.
Connan, Solène, 1976- editor.
ISBN 9781493926848