| Description |
1 online resource (xiii, 508 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 80 |
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Methods in molecular biology (Clifton, N.J.) ; v. 80.
|
| Contents |
Production of polyclonal antisera / Jonathan A. Green, Margaret M. Manson -- Raising polyclonal antibodies using nitrocellulose-bound antigen / Monique Diano, André Le Bivic, Michel Hirn -- Production and characterization of antibodies against synthetic peptides / David C. Hancock, Gerard I. Evan -- Preparation and testing of monoclonal antibodies to recombinant proteins / Christopher J. Dean -- A simple hollow-fiber bioreactor for the "in-house" production of monoclonal antibodies / Margaret Goodall -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase / Yveline Frobert, Jacques Grassi -- Synthesis of peptides for use as immunogens / David C. Hancock, Nicola J. Reilly, Gerard I. Evan -- Use of proteins blotted to polyvinylidene difluoride membranes as immunogens / Boguslaw Szewczyk, Donald F. Summers -- Purification of glycoproteins and their use as immunogens / Boguslaw Szewczyk, Donald F. Summers -- IgG purification / Mark Page, Robin Thorpe -- Purification of monoclonal antibodies / Mark Page, Robin Thorpe -- Production of bispecific and trispecific F(ab)₂ and F(ab)₃ antibody derivatives / Ruth R. French -- Preparation of cytotoxic antibody-toxin conjugates / Alan J. Cumber, Edward J. Wawrzynczak -- Immunoaffinity purification and quantification of antibody-toxin conjugates / Edward J. Wawrzynczak, Alan J. Cumber -- Competitive ELISA / Kittima Makarananda, Lucinda R. Weir, Gordon E. Neal -- Epitope mapping / Glenn E. Morris -- Coupling of antibodies with biotin / Rosaria P. Haugland, Wendy W. You -- Preparation of avidin conjugates / Rosaria P. Haugland, Mahesh K. Bhalgat -- Enhanced chemiluminescence immunoassay / Richard A. Stott -- Nonradioactive methods for visualization of protein blots / Michael Finney -- Colloidal gold staining and immunoprobing on the same Western blot / Denise Egger, Kurt Bienz -- Erasure of Western blots after autoradiographic or chemiluminescent detection / Scott H. Kaufmann, Udo Kellner -- Colloidal gold staining and immunodetection in 2D protein mapping / Anthony H. Schapira -- Enzyme-antienzyme method for immunohistochemistry / Michael G. Ormerod, Elizabeth Philp, Susanne F. Imrie -- Immunohistochemical detection of bromodeoxyuridine-labeled nuclei for in vivo cell kinetic studies / Jonathan A. Green, Richard E. Edwards, Margaret M. Manson -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine / George D. Wilson -- Immunohistochemical detection of cells in the division cycle using antibodies to proliferating cell nuclear antigen (PCNA) / Richard E. Edwards, Jerry Styles -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates / Jonathan A. Green, Margaret M. Manson -- Preparation of colloidal gold probes / David A. Hughes, Julian E. Beesley -- Immunogold probes in light microscopy / David A. Hughes, Julian E. Beesley -- Immunogold probes in electron microscopy / David J. Ferguson, David A. Hughes, Julian E. Beesley -- Electron microscopic-silver enhancement for double labeling with antibodies raised in the same species / Kurt Bienz, Denise Egger -- Flow cytometric analysis of cell surface antigen density / R. Adrian Robins -- Multiple immunofluorescence analysis of cells using flow cytometry / R. Adrian Robins -- Flow cytometric methods of analyzing apoptotic cells / Anne E. Milner, J. Michaela Levens, Christopher D. Gregory -- Flow cytometric detection of proliferation-associated antigens, PCNA and Ki-67 / George D. Wilson -- Sorting of human peripheral blood T-cell subsets using immunomagnetic beads / Eddie C.Y. Wang -- Preparation and use of nonradioactive hybridization probes / Victor T.-W. Chan -- Cellular human and viral DNA detection by nonisotopic in situ hybridization / C.S. Herrington -- Biotinylated probes in colony hybridization / Michael J. Haas -- Nonisotopic in situ hybridization : gene mapping and cytogenetics / Bhupendra Bhatt, Tulin Sahinoglu, Cliff Stevens -- Expression of recombinant antibody fusion proteins in E. coli / Stephen M. Hobbs -- Expression of antibody fusion proteins in mammalian cells / Anita A. Hamilton, John R. Adair, Simon J. Forster -- Antibody screening of bacteriophage [lambda] gt-11 DNA expression libraries / Peter Jones -- Display of antibody chains on filamentous bacteriophage / Peter Jones -- Production of human Fab antibody fragments from phage display libraries / J. Mark Hexham -- Antibodies from phage display libraries as immunochemical reagents / Dario Neri, Alessandro Pini, Ahuva Nissim |
| Summary |
This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Immunochemistry -- Methodology
|
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Antibodies -- isolation & purification
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Immunochemistry -- methods
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SCIENCE -- Chemistry -- Clinical.
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MEDICAL -- Clinical Medicine.
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Immunochemistry -- Methodology
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| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Pound, John D
|
| ISBN |
9781592592579 |
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1592592570 |
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0585246203 |
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9780585246208 |
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9781489942456 |
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1489942459 |
|
