| Description |
1 online resource (1 PDF file (xi, 363 pages)) : illustrations (chiefly color) |
| Series |
Methods in molecular biology, 1940-6029 ; 2501 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; v. 2501. 1064-3745
|
|
Springer protocols (Series) 1949-2448
|
| Contents |
Microbial Rhodopsins -- Molecular Biology of Microbial Rhodopsins -- Rhodopsin-Based Optogenetics: Basics and Applications -- Searching Metagenomes for New Rhodopsins -- E. coli Expression and Purification of Microbial and Viral Rhodopsins -- Crystallization of Microbial Rhodopsins -- Crystallographic Studies of Rhodopsins: Structure and Dynamics -- Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins -- Solid-State NMR Spectroscopy on Microbial Rhodopsins -- FTIR and Raman Spectroscopy of Rhodopsins -- Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins -- Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and [delta]pH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches -- Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments -- Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications -- Optogenetic Studies of Mitochondria -- In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins -- Optogenetic Control of Human Stem Cell-Derived Neurons |
| Summary |
This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Description based on online resource; title from PDF title page (SpringerLink, viewed February 9, 2023) |
| Subject |
Rhodopsin -- Laboratory manuals
|
|
Rhodopsin
|
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Rhodopsin
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Gordeliy, Valentin, editor
|
| ISBN |
9781071623299 |
|
107162329X |
|
