| Description |
1 online resource (xi, 247 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1442 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1442
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Springer protocols (Series)
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| Contents |
Human respiratory syncytial virus : an introduction / Patricia A. Jorquera, Lydia Anderson, and Ralph A. Tripp -- RSV growth and quantification by microtitration and qRT- PCR assays / Hayat Caidi, Jennifer L. Harcourt, and Lia M. Haynes -- Quantification of RSV infectious particles by plaque assay and immunostaining assay / Patricia A. Jorquera and Ralph A. Tripp -- Detection of RSV antibodies in human plasma by enzyme immunoassays / Samadhan J. Jadhao and Larry J. Anderson -- Secretory expression and purification of respiratory syncytial virus G and F proteins in human cells / Samadhan J. Jadhao and Larry J. Anderson -- Development of human monoclonal antibodies against respiratory syncytial virus using a high efficiency human hybridoma technique / Gabriela Alvarado and James E. Crowe Jr. -- Respiratory syncytial virus (RSV) : neutralizing antibody, a correlate of immune protection / Pedro A. Piedra, Anne M. Hause, and Letisha Aideyan -- Host factors modulating RSV infection : use of small interfering RNAs to probe functional importance / Leon Caly, Hong-mei Li, and David Jan -- In vitro modeling of RSV infection and cytopathogenesis in well-differentiated human primary airway epithelial cells (WD-PAECs) / Lindsay Broadbent ... [et at.] -- Reverse genetics of respiratory syncytial virus / Christopher C. Stobart [and others] -- Use of minigenome systems to study RSV transcription / Michael N. Teng and Kim C. Tran -- Screening for host factors directly interacting with RSV protein : microfluidics / Sarit Kipper [and others] -- Proteomic-based workflow using purified respiratory syncytial virus particles to identify cellular factors as drug targets / Tra Nguyen Huong, Boon Huan Tan, and Richard J. Sugrue -- MicroRNA profiling from RSV-infected biofluids, whole blood, and tissue samples / Lydia Anderson, Patricia A. Jorquera, and Ralph A. Tripp -- Mouse and cotton rat models of human respiratory syncytial virus / Penny A. Rudd, Weiqiang Chen, and Suresh Mahalingam -- In vivo assessment of airway function in the mouse model / Azzeddine Dakhama and Erwin W. Gelfand -- Evaluation of the adaptive immune response to respiratory syncytial virus / Cory J. Knudson [and others] |
| Summary |
Annotation This volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from pdf of title page (SpringerLink, viewed August 1, 2016) |
| Subject |
Respiratory syncytial virus -- Laboratory manuals
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Respiratory infections -- Laboratory manuals
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Respiratory Syncytial Virus, Human
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Infectious & contagious diseases.
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Medical -- Infectious Diseases.
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Respiratory infections
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Respiratory syncytial virus
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Tripp, Ralph A., editor.
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Jorquera, Patricia A., editor
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| ISBN |
9781493936878 |
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1493936875 |
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1493936859 |
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9781493936854 |
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