| Description |
1 online resource (x, 307 pages) : illustrations |
| Series |
Methods in molecular medicine ; 84 |
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Methods in molecular medicine ; 84. 1543-1894
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| Contents |
Molecular cloning of opioid receptors by cDNA library screening / Y.X. Pan -- Expression of opioid receptors in mammalian cell lines / Y.X. Pan -- Assessing opioid regulation of adenylyl cyclase activity in intact cells / D.R. Thakker, H.Z. Ozsoy and K.M. Standifer -- Analysis of opioid-induced kinase activation / L. Ma -- Study of opioid receptor phosphorylation using cell-labeling method with 32P-orthrophosphate / J.B. Wang -- Opioid receptor coupling to GIRK channels. In vitro studies using a Xenopus oocyte expression system and in vivo studies on weaver mutant mice / K. Ikeda [and others] -- Identification of alternatively spliced variants from opioid receptor genes / Y.X. Pan -- Immunohistochemical localization of mu-, delta- and kappa-opioid receptors within the antinociceptive brainstem circuits / A.E. Kalyuzhny -- In situ hybridization in neural tissues / H.B. Gutstein -- Quantitative single-cell RT-PCR for opioid receptors and housekeeping genes / S.C. Silbert -- Gene arrays and proteomics. A primer / L. Mouledous and H.B. Gutstein -- Opioid receptor oligomerization. Detection and functional characterization of interacting receptors / I. Gomes, J. Filipovska and L.A. Devi -- Recombinant opioid receptors. Structure-function relationship / J. Filipovska [and others] -- Receptor knock-out and gene targeting. Generation of knock-out mice / I. Sora, K. Ikeda and Y. Mishina -- Pharmacological interventions at the spinal cord. Intrathecal injections / D.A. Robinson and M. Zhuo -- Opioid tolerance in adult and neonatal rats / Z.Z. Pan -- Animal models of neuropathic pain / W.J. Martin, L.S. Stewart and J.W. Tarpley -- Place conditioning to study drug reward and aversion / W.A. Carlezon, Jr. -- Opiate self-administration / Z.X. Xi and E.A. Stein -- Acute, chronic, and cancer pain. Clinical management / A.W. Burton -- Clinical treatment of opioid addiction and dependence / W. Ling, R.A. Rawson and M. Compton |
| Summary |
Opioid research has grown enormously over the last decade, and with it, the need to apply multiple techniques from a variety of disciplines. In Opioid Research: Methods and Protocols, expert researchers and physicians provide a comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research, covering topics from molecular and genetic techniques, to behavioral analyses of animal models, to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages, in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction. Each proven technique contains step-by-step instructions, explanations of the basic science involved, and notes on avoiding pitfalls. Comprehensive and authoritative, Opioid Research: Methods and Protocols offers researchers an indispensable guide to all the major multidisciplinary techniques needed for successful research in opioid biology and medicine today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Opioids -- Laboratory manuals
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Narcotics -- metabolism
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Receptors, Opioid -- physiology
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MEDICAL -- Pain Medicine.
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MEDICAL -- Psychiatry -- Psychopharmacology.
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Opioids
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Opioïden.
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Pan, Zhizhong Z.
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| ISBN |
9781592593798 |
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1592593798 |
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1280842563 |
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9781280842566 |
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9786610842568 |
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6610842566 |
|
