880-01 Intro; Contents; List of contributors; Abbreviations; 1. Engineered growth factors and receptors, and their applications; 1. Introduction; Known growth factors and receptors; Modes of factor-receptor interaction; 2. Applications of engineered growth factors and receptors; 3. Principal methods for engineering growth factors and their receptors; Mutagenesis; Formation of chimeric proteins; Manufacture of alternative ligands and receptors; 4. Design of engineered growth factors and receptors; DNA sequence of factor or receptor to be engineered
880-01/(S Construction and expression of EGF/TGFα chimeras
Analysis of existing DNA sequence for useful restriction sitesDesign of wholly synthetic growth factors and receptors; 5. Expression of DNAs encoding growth factors or receptors; Deciding on an expression system; Microbial expression; In vitro expression; Eukaryotic gene expression; Acknowledgements; References; 2. Engineering of novel neurotrophins; 1. Introduction; 2. Structure-function analyses of neurotrophins; 3. Site-directed mutagenesis; Kunkel method; PCR-based mutagenesis; 4. Expression of recombinant protein; COS cell expression; Baculovirus expression
Summary
Growth factors are extra-cellular molecules that interact with receptors on the cell surface to initiate a sequence of events culminating in transcription of DNA prior to cell division. Disturbances in the production of growth factors or the sensitivity of their receptors are important in tumour growth. Therefore, there is much interest in engineering both growth factors and their receptors to intervene in cancer. This book describes a variety of techniques for theengineering, analysis, definition of function and detection of gene expression of growth factors and their receptors. A number of p
