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Book Cover
E-book

Title The nuclear receptor superfamily : methods and protocols / edited by Iain J. McEwan
Published New York : Humana Press, ©2009

Copies

Description 1 online resource (x, 281 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 505
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 505.
Springer protocols (Series)
Contents Nuclear receptors : one big family / Iain J. McEwan -- Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson -- Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg -- X-ray crystallography of agonis/antagonist-bound receptors / Ashley C.W. Pike -- FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen [and others] -- Receptor-DNA interactions : EMSA and footprinting / Jason T. Read [and others] -- Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills -- Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox -- High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky -- Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery -- Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli -- Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan -- Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra [and others] -- Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven -- Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi
Summary Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study
Analysis receptoren
receptors
celkernen
nuclei
moleculaire genetica
molecular genetics
moleculaire biologie
molecular biology
biologische technieken
biological techniques
protocollen
protocols
Molecular Genetics
Moleculaire genetica
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Nuclear receptors (Biochemistry)
Receptors, Cytoplasmic and Nuclear -- physiology
Receptors, Cytoplasmic and Nuclear
Nuclear receptors (Biochemistry)
Récepteur cytoplasmique et nucléaire.
Récepteurs nucléaires (biochimie)
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author McEwan, I. J
ISBN 9781603275750
1603275754