| Description |
1 online resource : illustrations |
| Series |
Methods in molecular biology ; v. 425 |
|
Methods in molecular biology (Clifton, N.J.) ; volume 425
|
| Contents |
Application of fluorescence dye saturation labeling for differential proteome analysis of 1, 000 microdissected cells from pancreatic ductal adenocarcinoma precursoe lesions / Barbara Sitek [and others] -- Albumin and immunoglobulin depletion of human plasma / Rosalind e. Jenkins [and others] -- Multi-component immunoaffinity subtraction and reversed-phase chromatography of human serum / James Martosella and Nina Zolotarjova -- Immunoaffinity fractionation of plasma proteins by chicken IgY antibodies / Lei Huang and Xiangming Fang -- Proteomics of cerebrospinal fluid methods for sample processing / John E. Hale [and others] -- Sample preparation of bronchoalveolar lavage fluid / Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez -- Preparation of nasal secretions for peoteome analysis / Begona Casado, Paolo Iadarola, and Lewis K. Pannell -- Preparation of urine samples for proteomic analysis / Rembert Pieper -- Isolation of cytoplasmatic proteins from cultures cells for two-dimensional gel electrophoresis / Ying Wang, Jen-Fu Chiu, and Qing-Yu He -- Sample preparation of culture medium from Madin-Darby Canine Kidney cells / Daniel Ambort, Daniel Lottaz, and Erwin Sterchi -- Sample preparation for mass spectromentry analysis of formalin-fixed paraffin-embedded tissue proteomic analysis of formalin-fixed tissue / Nicolas A. Stewart and Timothy D. Veenstra -- Metalloproteomics in the molecular study of cell physiology and disease / Hermann-Josef Thierse, Stefanie Helm, and Patrick Pankert -- Protein extraction from green plant tissue / Ragnar Flengsrud -- The terminator a device for high-throughput extraction of plant material / B.M. van den Berg -- -- Isolation of mitochondria from plant cell culture / Etienne H. Meyer and A. Harvey Millar -- Isolation and preparation of choroplasts from Arabidopsis thaliana plants / Sybille E. Kubis, Kathryn S. Lilley, and Paul Jarvis -- Isolation of plant cell wall proteins / Elisabeth Jamet [and others] -- Isolation and fractionation of the endoplasmic reticulum from castor bean (Ricinius communis) endosperm for proteomic analyses / William J. Simon, Daniel J. Maltman, and Antoni R. Slabas -- Cell wall fractionation for yeast and fungal proteomics / Aida Pitarch, Césa Nombela, and Concha Gil -- Sample preparation procedure for cellular fungi / Alois Harder -- Isolation and enrichment of secreted proteins from filamentous fungi / Martha L. Medina and Wilson A. Frnacisco -- Isolation and solubilization of cellular membrane proteins from bacteria / Kheir Zuobi-Hasona and L. Jeannine Brady -- Isolation and solubilization of gram-positive bacterial cell wall-associated proteins / Jason N. Cole, Steven P. Djordjevic, and Mark J. Walker -- Cell fractionation of parasitic protozoa / Wanderley de Souza, José Andrés-Morgado-Diaz, and Narcisa L. Cunha-e-Silva |
| Summary |
The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field |
| Analysis |
genexpressie |
|
gene expression |
|
page |
|
fractionering |
|
fractionation |
|
elektroforese |
|
electrophoresis |
|
eiwitten |
|
proteins |
|
monsterverwerking |
|
sample processing |
|
biochemie |
|
biochemistry |
|
biologische technieken |
|
biological techniques |
|
eiwitexpressieanalyse |
|
proteomics |
|
protocollen |
|
protocols |
|
Proteomics |
|
Molecular Biology (General) |
|
Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Proteomics.
|
|
Two-dimensional electrophoresis.
|
|
Sample preparation (Chemistry)
|
|
Proteomics -- methods
|
|
Analytic Sample Preparation Methods
|
|
Chemical Fractionation -- methods
|
|
Electrophoresis, Gel, Two-Dimensional -- methods
|
|
Proteomics
|
|
Electrophoresis, Gel, Two-Dimensional
|
|
Proteomics -- methods.
|
|
Analytic Sample Preparation Methods.
|
|
Chemical Fractionation -- methods.
|
|
Electrophoresis, Gel, Two-Dimensional -- methods.
|
|
Proteomics.
|
|
Two-dimensional electrophoresis.
|
|
Sample preparation (Chemistry)
|
|
Proteomics
|
|
Two-dimensional electrophoresis
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Posch, Anton.
|
| ISBN |
9781603272100 |
|
1603272100 |
|
