Title Yeast functional genomics and proteomics : methods and protocols / edited by Igor Stagljar

Published Dordrecht ; New York : Humana Press, ©2009

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Description 1 online resource (xiii, 300 pages, 4 unnumbered pages of plates) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 548 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 548 Springer protocols (Series) Contents Comparative genome hybridization on tiling microarrays to detect aneuploidies in yeast / Barry Dion and Grant W. Brown -- Identification of transcription factor targets by phenotypic activation and microarray expression profiling in yeast / Gordon Chua -- SGAM: an array-based approach for high-resolution genetic mapping in Saccharomyces cerevisiae / Michael Costanzo and Charles Boone -- Reporter-based synthetic genetic array analysis: a functional genomics approach for investigating the cell cycle in Saccharomyces cerevisiae / Holly E. Sassi [and others] -- The fidgety yeast: focus on high-resolution live yeast cell microscopy / Heimo Wolinski, Klaus Natter, and Sepp D. Kohlwein -- A genomic approach to yeast chronological aging / Christopher R. Burtner, Christopher J. Murakami, and Matt Kaeberlein -- Chemogenomic approaches to elucidation of gene function and genetic pathways / Sarah E. Pierce [and others] -- Identification of inhibitors of chromatin modifying enzymes using the yeast phenotypic screens / Benjamin Newcomb and Antonio Bedalov -- Exploiting yeast genetics to inform therapeutic strategies for Huntington's disease / Flaviano Giorgini and Paul J. Muchowski -- Global proteomic analysis of Saccharomyces cerevisiae identifies molecular pathways of histone modifications / Jessica Jackson and Ali Shilatifard -- Systematic characterization of the protein interaction network and protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry / Mohan Babu [and others] -- Protein microarrays / Joseph Fasolo and Michael Snyder -- Analysis of protein-protein interactions using array-based yeast two-hybrid screens / Seesandra V. Rajagopala and Peter Uetz -- Analysis of membrane protein complexes using the split-ubiquitin membrane yeast two-hybrid (MYTH) system / Saranya Kittanakom [and others] -- Computational analysis of the yeast proteome: understanding and exploiting functional specificity in genomic data / Curtis Huttenhower [and others] Summary Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. The chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls Analysis gisten yeasts saccharomyces cerevisiae methodologie methodology functionele genomica functional genomics protocollen protocols eiwitexpressieanalyse proteomics Fungi Schimmels Bibliography Includes bibliographical references and index Notes Print version record Subject Saccharomyces cerevisiae -- Genetics Molecular genetics -- Technique Genetics -- Technique. Saccharomyces cerevisiae -- genetics Genetic Techniques Genomics -- methods Laboratory Techniques and Procedures Proteomics -- methods Genetics -- Technique Molecular genetics -- Technique Saccharomyces cerevisiae -- Genetics Techniques génétiques. Génomique. Techniques et procédures de laboratoire. Protéomique. Hefeartige Pilze Genanalyse Untersuchungsmethode Proteomanalyse Saccharomyces cerevisiae. Génétique moléculaire. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Stagljar, Igor. ISBN 9781597455404 1597455407

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