| Description |
1 online resource (xii, 441 pages) : illustrations |
| Series |
Springer protocols |
|
Methods in molecular biology, 1064-3745 ; 564 |
|
Springer protocols (Series)
|
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Methods in molecular biology (Clifton, N.J.) ; v. 564
|
| Contents |
Introduction to proteomics / Friedrich Lottspeich -- High-resolution two-dimensional electrophoresis / Walter Weiss and Angelika Görg -- Non-classical 2-D electrophoresis / Jacqueline Burré, Ilka Wittig, and Hermann Schägger -- Protein detection and quantitation technologeis for gel-based proteome analysis / Walter Weiss, Florian Weiland, and Angelika Görg -- MALDI MS / Rainer Cramer -- Capillary electrophoresis coupled to mass spectrometry for proteomic profiling of human urine and biomarker discovery / Petra Zürbig, Eric Schiffer, and Harald Mischak -- A newcomer's guide to nano-liquid-chromatography of peptides / Thomas Fröhlich and Georg J. Arnold -- Multidimensional protein identification technology / Katharina Lohrig and Dirk Wolters -- Characterization of platelet proteins using peptide centric proteomics / Oliver Simon, Stefanie Wortelkamp, and Albert Sickmann -- Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis / Reinhold Weber [and others] -- Liquid chromatography-mass spectrometry-based quantitative proteomics / Michael W. Linscheid [and others] -- iTRAQ-labeling of in-gel digested proteins for relative quantification / Carla Schmidt and Henning Urlaub -- Electrospray mass spectrometry for quantitative plasma proteome analysis / Hong Wang and Sam Hannash -- Algorithms and databases / Lennart Martens and Rolf Apweiler -- Shotgun protein identification and quantification by mass spectrometry / Bingwen Lu [and others] -- Proteomics identification of oxidatively modified proteins in brain / Rukhsana Sultana, Marzia Perluigi, and D. Allan Butterfield -- Isotope-labeling and affinity enrichment of phosphopeptides for proteomic analysis using liquid chromatography-tandem mass spectrometry / Uma Kota, Ko-yi Chien, and Michael B. Goshe -- Organelle proteomics : reduction of sample complexity by enzymatic in-gel selection of native proteins / Veronika Reisinger and Lutz A. Eichacker -- Isolation of plasma membranes from the nervous system by countercurrent distribution in aqueous polymer two-phase systems / Jens Schindler and Hans Gerd Nothwang -- Enrichment and preparation of plasma membrane proteins from Arabidopsis thaliana for global proteomic analysis using liquid chromatography-tandem mass spectrometry / Srijeet K. Mitra, Steven D. Clouse, and Michael B. Goshe -- Tandem affinity purification of protein complexes from mammalian cells by the strep/FLAG (SF)-TAP tag / Christian Johannes Gloeckner [and others] -- Sequential peptide affinity pruification system for the systematic isolation and identification of protein complexes from Escherichia coli / Mohan Babu [and others] -- Bioinformatical approaches to detect and analyze protein interactions / Beate Kruger and Thomas Dandekar |
| Summary |
Since the development of the concept of "proteomics," great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research |
| Analysis |
eiwitten |
|
proteins |
|
peptiden |
|
peptides |
|
genexpressie |
|
gene expression |
|
biologische technieken |
|
biological techniques |
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eiwitexpressieanalyse |
|
proteomics |
|
protocollen |
|
protocols |
|
Proteomics |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Proteomics.
|
|
Proteins -- Analysis.
|
|
Proteomics
|
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Proteins -- Analysis
|
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Proteomics
|
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Protéomique.
|
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Protéomique.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Reinders, Jörg, 1976-
|
|
Sickmann, Albert.
|
| ISBN |
1607611570 |
|
9781607611578 |
|
