| Description |
1 online resource (xii, 477 pages) : illustrations (chiefly color) |
| Series |
Methods in molecular biology ; 1871 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1871. 1064-3745
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| Contents |
Making the case for functional proteomics / Ray C. Perkins -- Methods to monitor the functional subproteomes of SERPIN protease inhibitors / Swapan Roy and Matthew Kuruc -- Two-dimensional 16-BAC/SDS polyacrylamide gel electrophoresis of mitochondrial membrane proteins / Gary Smejkal and Srikanth Kakumanu -- Systematic glycolytic enzyme activity analysis from human serum with PEP technology / David Wang -- Protein decomplexation strategy in snake venom proteomics / Choo Hock Tan, Kae Yi Tan, and Nget Hong Tan -- Fractionation techniques to increase plant proteome coverage : combining separation in parallel at the protein and the peptide level / Martin Cerny, Miroslav Berka, and Hana Habanova -- Systematic analysis workflow for high-density customized protein microarrays in biomarker screening / Rodrigo Garcıa-Valiente, Jonatan Fernandez-Garcıa, Javier Carabias-Sanchez, Alicia Landeira-Vinuela, Rafael Gongora, Maria Gonzalez-Gonzalez, and Manuel Fuentes -- Metaproteomics study of the gut microbiome / Lisa A. Lai, Zachary Tong, Ru Chen, and Sheng Pan -- Double one-dimensional electrophoresis (D1-DE) adapted for immunoproteomics / Youcef Shahali, Helene Senechal, and Pascal Poncet -- BioID : a proximity-dependent labeling approach in proteomics study / Peipei Li, Yuan Meng, Li Wang, and Li-jun Di -- Functional application of snake venom proteomics in in vivo antivenom assessment / Choo Hock Tan and Kae Yi Tan -- Proteomic detection of carbohydrate-active enzymes (CAZymes) in microbial secretomes / Tina R. Tuveng, Vincent G.H. Eijsink, and Magnus Ø. Arntzen -- Overview of mass spectrometry-based methods for functional proteomics / J. Robert O'Neill -- Functional proteomic analysis to characterize signaling crosstalk / Sneha M. Pinto, Yashwanth Subbannayya, and T.S. Keshava Prasad -- Identification of unexpected protein modifications by mass spectrometry-based proteomics / Shiva Ahmadi and Dominic Winter -- Label-free LC-MS/MS strategy for comprehensive proteomic profiling of human islets collected using laser capture microdissection from frozen pancreata / Lina Zhang, Giacomo Lanzoni, Matteo Battarra, Luca Inverardi, and Qibin Zhang -- Targeted proteomics / Yun Chen and Liang Liu -- Metabolomic investigation of Staphylococcus aureus antibiotic susceptibility by liquid chromatography coupled to high-resolution mass spectrometry / Sandrine Aros-Calt, Florence A. Castelli, Patricia Lamourette, Gaspard Gervasi, Christophe Junot, Bruno H. Muller, and Francois Fenaille -- Nuts and bolts of protein quantification by online trypsin digestion coupled LC-MS/MS analysis / Christopher A. Toth, Zsuzsanna Kuklenyik, and John R. Barr -- Proteases : pivot points in functional proteomics / Ingrid M. Verhamme, Sarah E. Leonard, and Ray C. Perkins -- Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics / Youcef Shahali, Helene Senechal, and Pascal Poncet -- Efficient extraction and digestion of gluten proteins / Haili Li, Keren Byrne, Crispin A. Howitt, and Michelle L. Colgrave -- Glycosylation profiling of tumor marker in plasma using bead-based immunoassay / Hongye Wang, Zheng Cao, Hu Duan, and Xiaobo Yu -- Protein-specific analysis of invertebrate glycoproteins / Alba Hykollari, Daniel Malzl, Iain B.H. Wilson, and Katharina Paschinger -- Use of proteomics studies in identifying moonlighting proteins / Constance Jeffery -- Two-dimensional biochemical purification for global proteomic analysis of macromolecular protein complexes / Reza Pourhaghighi and Andrew Emili -- Data analysis protocol for quantitative data-independent acquisition proteomics / Sami Pietil€a, Tomi Suomi, Juhani Aakko, and Laura L. Elo |
| Summary |
This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink viewed October 1, 2018) |
| Subject |
Proteomics -- Methodology -- Laboratory manuals
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Proteomics -- methods
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Laboratory Manuals
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Wang, Xing, editor
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|
Kuruc, Matthew, editor
|
| ISBN |
9781493988143 |
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149398814X |
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