Title Phospho-proteomics : methods and protocols / edited by Marjo de Graauw

Published New York, NY : Humana Press, ©2009

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Description 1 online resource (xi, 328 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 527 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 527 Springer protocols (Series) Contents A high-resolution two dimensional gel- and pro-Q DPS-based proteomics workflow for phosphoprotein identification and quantitative profiling / G.K. Agrawal and J.J. Thelen -- Differential phosphoprotein labelling (DIPPL) Using ³²P and ³³P / A.M. Tolkovsky and A. Wyttenbach -- Identification of oxidative stress-induced tyrosine phosphorylated proteins by immunoprecipitation and mass spectrometry / M.C. Duran, H.L. Chan and J.F. Timms -- Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography (IMAC) and mass spectrometry / T.E. Thingholm and O.N. Jensen -- The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests / T.E. Thingholm and M.R. Larsen -- Enrichment and separation of mono- and multiply phosphorylated peptides using sequential elution from IMAC prior to mass spectrometric analysis / T.E. Thingholm, O.N. Jensen and M.R. Larsen -- Use of stable isotope labeling by amino acids in cell culture (SILAC) for phosphotyrosine protein identification and quantitation / G. Zhang and T.A. Neubert -- Hydrophilic interaction chromatography for fractionation and enrichment of the phosphoproteome / D.E. McNulty and R.S. Annan -- SILAC for global phosphoproteomic analysis / G. Pimienta, R. Chaerkady and A. Pandey -- Quantitative phospho-proteomics based on soluble nanopolymers / A. Iliuk and W.A. Tao -- Profiling the tyrosine phosphorylation state using SH2 domains / K. Dierck [and others] -- An overview of the qualitative analysis of phosphoproteins by mass spectrometry / P.R. Gafken -- The analysis of phosphoproteomes by selective labelling and advanced mass spectrometric techniques / A. Amoresano [and others] -- On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins / S.M. Sweet and H.J. Cooper -- Quantification of protein phosphorylation by [mu]LC-ICP-MS / R. Kruger, N. Zinn and W.D. Lehmann -- Reverse-phase diagonal chromatography for phosphoproteome research / K. Gevaert and J. Vandekerckhove -- Chemical tagging strategies for mass spectrometry-based phospho-proteomics / A. Leitner and W. Lindner -- Antibody array platform to monitor protein tyrosine phosphorylation in mammalian cells / A.S. Chung and Y.E. Chin -- Protein tyrosine kinase characterization based on fully automated synthesis of (phospho)peptide arrays in microplates / W.C. Saxinger -- Kinome profiling using peptide arrays in eukaryotic cells / K. Parikh, M.P. Peppelenbosch and T. Ritsema -- ProMoST: A Tool for Calculating the pI and Molecular Mass of Phosphorylated and Modified proteins on two-dimensional gels / B.D. Halligan -- Kinase-specific prediction of protein phosphorylation sites / M.L. Miller and N. Blom -- Reconstructing regulatory kinase pathways from phosphopeptide data: a bioinformatics approach / L.G. Puente, R.E. Lee and L.A. Megeney Summary Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Protocols, expert researchers contribute both well-established protocols and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Protocols is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future Analysis phosphoproteins fosforylering phosphorylation etiketteren labelling biologische technieken biological techniques eiwitexpressieanalyse proteomics protocollen protocols Proteomics Bibliography Includes bibliographical references and index Notes English Print version record Subject Phosphorylation. Phosphoproteins. Proteomics. Protein kinases. Phosphoproteins Proteomics Protein Kinases Phosphorylation Proteomics -- methods Phosphoproteins Phosphorylation Protein kinases Proteomics Protéomique. Phosphorylation. Phosphore -- Métabolisme. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Graauw, Marjo de, 1978- ISBN 9781603278348 1603278346

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