Limit search to available items
Book Cover

Title Proteases and cancer : methods and protocols / edited by Santiago Cal and Alvaro J. Obaya
Published New York, N.Y. : Humana Press : Springer, [2018]


Description 1 online resource (xv, 352 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1731
Methods in molecular biology (Clifton, N.J.) ; v. 1731. 1064-3745
Contents Dissecting degradomes : analysis of protease-coding genes / Ángel Álvarez-Eguiluz, Ander Díaz-Navarro, and Xose S. Puente -- Identification of protease cleavage sites and substrates in cancer by Carboxy-TAILS (C-TAILS) / Nestor Solis and Christopher M. Overall -- Identification of proteolytic cleavage sites of EphA2 by membrane type 1 matrix metalloproteinase on the surface of cancer cells/ Keiji Kikuchi, Hiroko Kozuka-Hata, Masaaki Oyama, Motoharu Seiki, and Naohiko Koshikawa -- Biotin-chasing assay to evaluate uPAR stability and cleavage on the surface of cells / Vladimir Leksa, Herbert B. Schiller, and Hannes Stockinger -- Determination of aconitase activity : a substrate of the mitochondrial lon protease / Pedro M. Quirós -- Simple cell-based assay for the detection of surface protein shedding by rhomboid proteases / Angela Moncada-Pazos and Adam Graham Grieve -- Functional production of catalytic domains of human MMPs in Escherichia coli periplasm / Dong Hyun Nam, Ki Baek Lee, and Xin Ge -- Autophagy and proteases : basic study of the autophagic flux by western blot / Álvaro F. Fernández -- Gel-based gelatin zymography to examine matrix metalloproteinase activity in cell culture / Aastha Chhabra and Vibha Rani -- Analysis of enzymatic activity of matrix metalloproteinase (MMP) by collagen zymography in melanoma / Vijay Walia and Yardena Samuels -- Measurement of protease activities using fluorogenic substrates/ Salvatore Santamaria and Hideaki Nagase -- Targeting the expression of cathepsin B using CRISPR/Cas9 system in mammalian cancer cells / Manu Gnanamony and Christopher S. Gondi -- Assessing the influence of a protease in cell migration using the barrier-migration assay / Tania Fontanil, Yamina Mohamedi, Santiago Cal, and Álvaro J. Obaya -- Analysis of invasive activity of CAF spheroids into three dimensional (3D) collagen matrices / María Ángeles Villaronga, Saúl Álvarez Teijeiro, Francisco Hermida-Prado, Marta Garzón-Arango, Victoria Sanz-Moreno, and Juana María García-Pedrero -- Beyond the proteolytic activity : examining the functional relevance of the ancillary domains using tri-dimensional (3D) spheroid invasion assay / Erico Tosoni Costa and Anamaria Aranha Camargo -- Analyzing the type II transmembrane serine protease hepsin-dependent basement membrane remodeling in 3D cell culture / Shishir M. Pant, Denis Belitskin, Hanna Ala-Hongisto, Juha Klefström, and Topi A. Tervonen -- Evaluation of tumor vasculature using a syngeneic tumor model in wild-type and genetically modified mice / Francisco Javier Rodríguez-Baena, Silvia Redondo-García, María del Carmen Plaza-Calonge, Rubén Fernández-Rodríguez, and Juan Carlos Rodríguez-Manzaneque -- 3D image analysis of the microvasculature in healthy and diseased tissues / Álvaro Sahún-Español, Cristina Clemente, and Alicia G. Arroyo -- Human tumor tissue-based 3D in vitro invasion assays / Pirjo Åström, Ritva Heljasvaara, Pia Nyberg, Ahmed Al-Samadi, and Tuula Salo -- Ear sponge assay : a method to investigate angiogenesis and lymphangiogenesis in mice / Maureen Van de Velde, Melissa García-Caballero, Tania Durré, Frédéric Kridelka, and Agnès Noël -- Cancer susceptibility models in protease-deficient mice / Alicia R. Folgueras, Sandra Freitas-Rodríguez, Yaiza Español, and Gloria Velasco -- Imaging proteolytic activities in mouse models of cancer / Anupama Pal and Alnawaz Rehemtulla -- Protease silencing to explore the molecular mechanisms of cancer and aging / Julia M. Fraile, Diana Campos-Iglesias, and José M.P. Freije -- Analysis of somatic DNA methylation alterations of genes encoding cell surface metallopeptidases in colorectal cancer / Sergio Alonso, Beatriz González, Andreu Alibés, and Manuel Perucho -- Targeting deubiquitinases in cancer / Joseph S. Bednash and Rama K. Mallampalli -- Generation of highly selective MMP antibody inhibitors / Dong Hyun Nam and Xin Ge -- Strategies to target matrix metalloproteinases as therapeutic approach in cancer / Zoi Piperigkou, Dimitra Manou, Konstantina Karamanou, and Achilleas D. Theocharis
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocols, viewed January 10, 2018)
Subject Proteolytic enzymes -- Laboratory manuals
Cancer -- Etiology -- Laboratory manuals
Peptide Hydrolases
Medical -- Oncology.
Proteolytic enzymes
Cancer -- Etiology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Cal, Santiago, editor
Obaya, Alvaro J., editor
ISBN 9781493975952