| Description |
1 online resource (xiv, 511 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 895 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 895. 1064-3745
|
| Contents |
Machine generated contents note: pt. I Assessing IDPs in the Living Cell -- 1. Determination of IUP Based on Susceptibility for Degradation by Default / Yosef Shaul -- 2. In-Cell NMR of Intrinsically Disordered Proteins in Prokaryotic Cells / Brian O. Smith -- 3. In-Cell NMR in Xenopus laevis Oocytes / Philipp Selenko -- 4. In-Cell NMR in Mammalian Cells: Part 1 / Philipp Selenko -- 5. In-Cell NMR in Mammalian Cells: Part 2 / Philipp Selenko -- 6. In-Cell NMR in Mammalian Cells: Part 3 / Philipp Selenko -- 7. Fourier Transform Infrared Microspectroscopy of Complex Biological Systems: From Intact Cells to Whole Organisms / Silvia Maria Doglia -- 8. Studying IDP Stability and Dynamics by Fast Relaxation Imaging in Living Cells / Martin Gruebele -- pt. II NMR-Based Techniques -- 9. Measurement and Analysis of NMR Residual Dipolar Couplings for the Study of Intrinsically Disordered Proteins / |
| Summary |
Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions. In Intrinsically Disordered Protein Analysis :Methods and Experimental Tools, expert researchers explore the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. Volume 1 includes sections on assessing IDPs in the living cell, NMR based techniques, vibrational spectroscopy, and other spectroscopic techniques. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Intrinsically Disordered Protein Analysis: Methods and Experimental Tools helps scientists further their investigations of these fascinating and dynamic molecules |
| Analysis |
Life sciences |
|
Biochemistry |
|
Protein Science |
|
Protein Structure |
|
eiwitten |
|
proteins |
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Proteins and Enzymes |
|
Eiwitten en enzymen |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Online resource; title from PDF title page (Springer Protocols, viewed Oct. 18, 2012) |
| Subject |
Proteins -- Laboratory manuals
|
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Proteins -- Denaturation -- Laboratory manuals
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Proteins -- Analysis -- Laboratory manuals
|
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Proteins -- Denaturation.
|
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Proteins.
|
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Protein Denaturation
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Proteins
|
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Proteins -- analysis
|
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protein.
|
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Proteins
|
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Proteins -- Analysis
|
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Proteins -- Denaturation
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Uversky, Vladimir N.
|
|
Dunker, A. Keith (Alan Keith), 1943-
|
| ISBN |
9781617799273 |
|
1617799270 |
|
1617799262 |
|
9781617799266 |
|
