| Description |
1 online resource (x, 279 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1216 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1216. 1064-3745
|
| Contents |
De novo design of stable [alpha]-helices / Alexander Yakimov, Georgy Rychkov, and Michael Petukhov -- Design of monomeric water-soluble [Beta]-hairpin and [Beta]-sheet peptides/ M. Angeles Jiménez -- Combination of theoretical and experimental approaches for the design and study of fibril-forming peptides / Phanourios Tamamis [and others] -- Posttranslational incorporation of noncanonical amino acids in the RNase S system by semisynthetic protein assembly / Maika Genz and Norbert Sträter -- Design, synthesis, and study of fluorinated proteins / Benjamin C. Buer and E. Neil G. Marsh -- High-quality combinatorial protein libraries using the binary patterning approach / Luke H. Bradley -- Methods for library-scale computational protein design / Lucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow -- Symmetric protein architecture in protein design : top-down symmetric deconstruction / Liam M. Longo and Michael Blaber -- Identification of protein scaffolds for enzyme design using scaffold selection / André C. Stiel, Kaspar Feldmeier, and Birte Höcker -- Computational design of novel enzymes without cofactors / Matthew D. Smith, Alexandre Zanghellini, and Daniela Grabs-Röthlisberger -- De novo design of peptide scaffolds as novel preorganized ligands for metal-ion coordination / Aimee J. Gamble and Anna F.A. Peacock -- Computational design of metalloproteins / Avanish S. Parmar, Douglas Pike, and Vikas Nanda -- Incorporation of modified and artificial cofactors into naturally occurring protein scaffolds / Koji Oohora and Takashi Hayashi -- Computational redesign of metalloenzymes for catalyzing new reactions / Per Jr. Greisen and Sagar D. Khare |
| Summary |
Protein Design: Method and Applications, Second Editionexpands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters onmetals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Protein Design: Method and Applications, Second Editionprovides a number of practical protocols and instructive reviews to aid in the creation of new experiments |
| Analysis |
eiwitten |
|
proteins |
|
levenswetenschappen |
|
life sciences |
|
computertechnieken |
|
computer techniques |
|
Proteins and Enzymes |
|
Eiwitten en enzymen |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Protein engineering.
|
|
Proteins -- Conformation.
|
|
Protein Engineering -- methods
|
|
Protein Conformation
|
|
Protein Folding
|
|
Proteins -- chemistry
|
|
Protein Engineering
|
|
Proteins -- Conformation
|
|
Protein engineering
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Kohler, Valentin
|
| LC no. |
2014947803 |
| ISBN |
9781493914869 |
|
1493914863 |
|
1493914855 |
|
9781493914852 |
|
