| Description |
1 online resource (xi, 311 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1588 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1588. 1064-3745
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| Contents |
Low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases / Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, and Harry Brumer -- Quantitative kinetic characterization of glycoside hydrolases using high-performance anion-exchange chromatography (HPAEC) / Nicholas McGregor, Gregory Arnal, and Harry Brumer -- Measuring enzyme kinetics of glycoside hydrolases using the 3,5-dinitrosalicylic acid assay / Lauren S. McKee -- Improved kinetic assay for the characterization of metal-dependent pectate lyases / Darryl R. Jones, Richard McLean, and D. Wade Abbott -- Colorimetric detection of acetyl xylan esterase activities / Galina Mai-Gisondi and Emma R. Master -- Methods for determining glycosyltransferase kinetics / Maria Ngo and Michael D.L. Suits -- Analyzing activities of lytic polysaccharide monooxygenases by liquid chromatography and mass spectrometry / Bjørge Westereng, Magnus Ø. Arntzen, Jane Wittrup Agger, Gustav Vaaje-Kolstad, and Vincent G.H. Eijsink -- Carbohydrate depolymerization by intricate cellulosomal systems / Johanna Stern, Lior Artzi, Sarah Moraïs, Carlos M.G.A. Fontes, and Edward A. Bayer -- Affinity electrophoresis for analysis of catalytic module-carbohydrate interactions / Darrell Cockburn, Casper Wilkens, and Birte Svensson -- Quantifying CBM carbohydrate interactions using microscale thermophoresis / Haiyang Wu, Cédric Y. Montanier, and Claire Dumon -- Characterization of protein-carbohydrate interactions by NMR spectroscopy / Julie M. Grondin, David N. Langelaan, and Steven P. Smith -- Measuring the biomechanical loosening action of bacterial expansins on paper and plant cell walls / Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, and Daniel M. Durachko -- Bioinspired assemblies of plant cell walls for measuring protein-carbohydrate interactions by FRAP / Gabriel Paës -- CBMs as probes to explore plant cell wall heterogeneity using immunocytochemistry / Louise Badruna, Vincent Burlat, and Cédric Y. Montanier -- Determining the localization of carbohydrate active enzymes within gram-negative bacteria / Richard McLean, G. Douglas Inglis, Steven C. Mosimann, Richard R.E. Uwiera, and D. Wade Abbott -- Analysis of complex carbohydrate composition in plant cell wall using fourier transformed mid-infrared spectroscopy (FT-IR) / Ajay Badhan, Yuxi Wang, and Tim A. McAllister -- Separation and visualization of glycans by fluorophore-assisted carbohydrate electrophoresis / Mélissa Robb, Joanne K. Hobbs, and Alisdair B. Boraston -- Rapid procedure for the purification of 8-aminopyrene trisulfonate (APTS)-labeled glycans for capillary electrophoresis (CE)-based enzyme assays / Hayden J. Danyluk, Leona K. Shum, and Wesley F. Zandberg -- Probing the complex architecture of multimodular carbohydrate-active enzymes using a combination of small angle x-ray scattering and x-ray crystallography / Mirjam Czjzek and Elizabeth Ficko-Blean -- Metagenomics and CAZyme discovery / Benoit J. Kunath, Andreas Bremges, Aaron Weimann, Alice C. McHardy, and Phillip B. Pope -- Identification of genes involved in the degradation of lignocellulose using comparative transcriptomics / Robert J. Gruninger, Ian Reid, Robert J. Forster, Adrian Tsang, and Tim A. McAllister -- Isolation and preparation of extracellular proteins from lignocellulose degrading fungi for comparative proteomic studies using mass spectrometry / Robert J. Gruninger, Adrian Tsang, and Tim A. McAllister -- Eratum to : Colorimetric detection of acetyl xylan esterase activities / Galina Mai-Gisondi and Emma R. Master |
| Summary |
This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes: Part I describes methods for screening and quantifying CAZyme activity; Part II contains methods for investigating the interactions between proteins and carbohydrate ligands; Part III discusses methods for the visualization of carbohydrates and protein-carbohydrate complexes; and Part IV focuses on structural and "omic" approaches for studying systems of CAZymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein-Carbohydrate Interactions: Methods and Protocols is a valuable resource to the glycomics research community. In this continuously advancing field, the methods in this book highlight the biology of glycomics, thus driving biotechnological innovation and solutions for human health and sustainable resources within the emerging green community |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed April 19, 2017) |
| Subject |
Proteins -- Laboratory manuals
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Carbohydrates -- Laboratory manuals
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Carbohydrate Metabolism
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Carbohydrates -- chemistry
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Enzymes -- analysis
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Proteins -- chemistry
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Carbohydrates
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Proteins
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Abbott, D. Wade, 1974- editor
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Lammerts van Bueren, Alicia, editor
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| ISBN |
9781493968992 |
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1493968998 |
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149396898X |
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9781493968985 |
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