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Title Protein crystallography : methods and protocols / edited by Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski
Published New York, N.Y. : Humana Press : Springer, [2017]


Description 1 online resource (xiii, 672 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; v. 1607
Methods in molecular biology (Clifton, N.J.) ; v. 1607. 1064-3745
Contents Expression and purification of recombinant proteins in Escherichia coli with a His6 or dual His6-MBP tag / Sreejith Raran-Kurussi and David S. Waugh -- Protein crystallization / Alexander McPherson -- Advanced methods of protein crystallization / Abel Moreno -- "Sticky Patch" model of crystallization and modification of proteins for enhanced crystallizability / Zygmunt S. Derewenda and Adam Godzik -- Crystallization of membrane proteins : an overview / Andrii Ishchenko, Enrique E. Abola, and Vadim Cherezov -- Locating and visualizing crystals for x-ray diffraction experiments / Michael Becker, David J. Kissick, and Craig M. Ogata -- Collection of x-ray diffraction data from macromolecular crystals / Zbigniew Dauter -- Identifying and overcoming crystal pathologies : disorder and twinning / Michael C. Thompson -- Applications of x-ray micro-beam for data collection / Ruslan Sanishvili and Robert F. Fischetti -- Serial synchrotron x-ray crystallography (SSX) / Kay Diederichs and Meitian Wang -- Time-resolved macromolecular crystallography at modern x-ray sources / Marius Schmidt -- Structure determination using x-ray free-electron laser pulses / Henry N. Chapman -- Processing of XFEL Data / Thomas A. White -- Many ways to derivatize macromolecules and their crystals for phasing / Miroslawa Dauter and Zbigniew Dauter -- Experimental phasing : substructure solution and density modification as implemented in SHELX / Andrea Thorn -- Contemporary use of anomalous diffraction in biomolecular structure analysis / Qun Liu and Wayne A. Hendrickson -- Long-wavelength x-ray diffraction and its applications in macromolecular crystallography / Manfred S. Weiss -- Acknowledging errors : advanced molecular replacement with phaser / Airlie J. McCoy -- Rosetta structure prediction as a tool for solving difficult molecular replacement problems / Frank DiMaio -- Radiation damage in macromolecular crystallography / Elspeth F. Garman and Martin Weik -- Boxes of model building and visualization / Dušan Turk -- Structure refinement at atomic resolution / Mariusz Jaskolski -- Low resolution refinement of atomic models against crystallographic data / Robert A. Nicholls, Oleg Kovalevskiy, and Garib N. Murshudov -- Stereochemistry and validation of macromolecular structures / Alexander Wlodawer -- Validation of protein-ligand crystal structure models : small molecule and peptide ligands / Edwin Pozharski, Marc C. Deller, and Bernhard Rupp -- Protein data bank (PDB) : the single global macromolecular structure archive / Stephen K. Burley, Helen M. Berman, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, and Sameer Velankar -- Databases, repositories, and other data resources in structural biology / Heping Zheng, Przemyslaw J. Porebski, Marek Grabowski, David R. Cooper, and Wladek Minor
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed June 2, 2017)
Subject Proteins -- Laboratory manuals
Protein Interaction Mapping
Crystallography -- methods
Science -- Life Sciences -- Biochemistry.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Wlodawer, Alexander, editor
Dauter, Zbigniew, editor
Jaskolski, Mariusz, editor
ISBN 9781493970001