Title Therapeutic proteins : methods and protocols / edited by C. Mark Smales and David C. James

Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (xiv, 482 pages) : illustrations Series Methods in molecular biology ; 308 Methods in molecular biology (Clifton, N.J.) ; v. 308. Contents Biopharmaceutical proteins / John R. Birch and Yemi Onakunle -- Expression of antibody fragments by periplasmic secretion in Escherichia coli / Andrew G. Popplewell [and others] -- Secretory production of therapeutic proteins in Escherichia coli / Sang Yup Lee, Jong Hyun Choi, and Sang Jun Lee -- Expression of recombinant LTB protein in marine vibrio VSP60 / Wang Yili and Si Lusheng -- Heterologous gene expression in yeast / Lee J. Byrne, Kenneth J. O'Callaghan, and Mick F. Tuite -- Pharmaceutical proteins from methylotrophic yeasts / Eric C. de Bruin [and others] -- Expression of human papillomavirus type 16 L1 in baculovirus expression systems / Zheng Jin, Si Lusheng, and Wang Yili -- Large-scale transient expression of therapeutic proteins in mammalian cells / Sabine Geisse, Martin Jordan, and Florian M. Wurm -- Site-specific integration for high-level protein production in mammalian cells / Bhaskar Thyagarajan and Michele P. Calos -- Production of recombinant therapeutic proteins by mammalian cells in suspension culture / Lily Chu, Ilse Blumentals, and Gargi Maheshwari -- Transgene control engineering in mammalian cells / Beat P. Kramer and Martin Fussenegger -- Fusion to albumin as a means to slow the clearance of small therapeutic proteins / William P. Sheffield, Teresa R. McCurdy, and Varsha Bhakta -- High throughput recovery of therapeutic proteins from the inclusion bodies of Escherichia coli / Amulya K. Panda -- The isolation, solubilization, refolding, and chromatographic purification of recombinant human growth hormone from inclusion bodies of Escherichia coli cells / Amulya K. Panda, A.N.S. Eshwari, and Lalit C. Garg -- Large-scale preparation of factor VIIa from human plasma / Teruhisa Nakashima and Kazuhiko Tomokiyo -- Purification of clinical-grade monoclonal antibodies by chromatographic methods / Alberto L. Horenstein, Ilaria Durelli, and Fabio Malavasi -- Virus elimination and validation / Nicola Boschetti and Anna Johnston -- Virus removal by nanofiltration / Marina Korneyeva and Scott Rosenthal Determining residual host cell antigen levels in purified recombinant proteins by slot blot and scanning laser densitometry / Aaron P. Miles, Daming Zhu, and Allan Saul -- Principles of biopharmaceutical protein formulation / Scott P. Sellers and Yuh-Fun Maa -- Solid-state protein formulation / Yuh-Fun Maa and Scott P. Sellers -- Stabilization of therapeutic proteins by chemical and physical methods / Ken-ichi Izutsu -- Extraction and characterization of vaccine antigens from water-in-oil adjuvant formulations / Aaron P. Miles and Allan Saul -- Probing reversible self-association of therapeutic proteins by sedimentation velocity in the analytical ultracentrifuge / Bernardo Perez-Ramirez and John J. Steckert -- Biological characterization of pegylated interferons / Lei Xie [and others] -- Characterization of interferon alpha-2B pegylated via carboxyalkylation / David C. Wylie [and others] -- Quantifying recombinant proteins and their degradation products using SDS-PAGE and scanning laser densitometry / Aaron P. Miles and Allan Saul Summary With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation Bibliography Includes bibliographical references and index Notes English Print version record Subject Protein drugs -- Laboratory manuals Proteins. Proteins -- therapeutic use Proteins -- chemical synthesis Proteins -- isolation & purification Proteins Amino Acids, Peptides, and Proteins Chemicals and Drugs protein. MEDICAL -- Pharmacology. Proteins Protein drugs Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Smales, C. Mark James, David C. (David Cameron), 1963- LC no. 2004021242 ISBN 1588293904 9781588293909 9781592599226 1592599222 1280359099 9781280359095 9786610359097 6610359091

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