Title Co-immunoprecipitation methods for brain tissue / edited by Yuji Odagaki, Dasiel O. Borroto-Escuela

Published New York : Humana Press : Springer, [2019] ©2019

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Description 1 online resource (xiii, 166 pages) : illustrations (chiefly color) Series Neuromethods, 1940-6045 ; 144 Neuromethods ; v. 144. 0893-2336 Contents Co-immunoprecipitation methods for detection of G protein-coupled receptors in brain tissue / Kazunori Namba and Hiroki Kaneko -- Co-immunoprecipitation methods to identify associated proteins with estrogen receptor [alpha] at postsynaptic density in brain tissue / Gen Murakami and Suguru Kawato -- Use of co-immunoprecipitation to study conformation-specific protein interactions of oligomeric [alpha]-synuclein aggregates / Cristine Betzer, Rikke Hahn Kofoed, and Poul Henning Jensen -- Using co-immunoprecipitation and shotgun mass spectrometry for protein-protein interaction identification in cultured human oligodendrocytes / Bradley Smith, Daniel Martins-de-Souza, and Mariana Fioramonte -- Co- immunoprecipitation analysis of GPCR complexes in the central nervous system / Yuji Kamikubo and Takashi Sakurai -- Histoblot technique : a reliable approach to analyze expression profile of proteins and to predict their molecular association / Carolina Aguado and Rafael Luján -- Co-immunoprecipitation assay to investigate the interaction strength between synaptic proteins using COS-7 cells / Sosuke Yagishita -- Guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTP[gamma]S) binding/immunoprecipitation assay using magnetic beads coated with anti-g[alpha] antibody in mammalian brain membranes / Yuji Odagaki -- Co-immunoprecipitation as a useful tool for detection of G protein-coupled receptor oligomers / Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, and Alicia Rivera -- Isolation and detection of G protein-coupled receptor (GPCR) heteroreceptor complexes in rat brain synaptosomal preparation using a combined brain subcellular fractionation/co-immunoprecipitation (Co-IP) procedures / Dasiel O. Borroto-Escuela, Manuel Narvaez, Martina Zannoni, Chiara Contri, Minerva Crespo-Ramírez, Michael di Palma, Patrizia Ambrogini, Daily Y. Borroto-Escuela, Ismel Brito, Mariana Pita-Rodríguez, Ismael Valladolid-Acebes, Miguel Pérez de la Mora, and Kjell Fuxe -- Co-immunoprecipitation of membrane-bound receptors from subsynaptic compartments / Wilber Romero-Fernandez, Maria Garcia-Barcelo, and Yunis Perez-Betancourt -- Coimmunoprecipitation (co-IP) analysis for protein-protein interactions in the neurons of the cerebral ganglia of the land snails of the genus Polymita during aestivation / Daily Y. Borroto-Escuela, Idania Hernández-Ramos, Kjell Fuxe, and Dasiel O. Borroto-Escuela -- Co-immunoprecipitation (Co-IP) of G protein-coupled receptor (GPCR)-receptor tyrosine kinase (RTK) complexes from the dorsal hippocampus of the rat brain / Michael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini, Kjell Fuxe, Patrizia Ambrogini, and Dasiel O. Borroto-Escuela Summary "This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory"--Publisher's description Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed December 20, 2018) Subject Brain -- Immunology -- Laboratory manuals Brain. Immune complexes. Immunoprecipitation -- methods Brain -- cytology Immunoprecipitation Brain Laboratory Manuals Protein Interaction Mapping -- methods Receptors, G-Protein-Coupled -- analysis Receptor Protein-Tyrosine Kinases -- analysis Antigen-Antibody Complex brains. Immune complexes Brain Form Electronic book Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Author Odagaki, Yuji, editor Borroto-Escuela, Dasiel O., editor ISBN 9781493989850 1493989855

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