Title Cell-free protein synthesis : methods and protocols / edited by Kirill Alexandrov, Wayne A. Johnston

Published New York : Humana Press, 2014

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xi, 313 pages) : illustrations (some color) Series Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1118 Methods in molecular biology (Clifton, N.J.) ; v. 1118. 1064-3745 Contents Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae -- Bioinformatics Analysis and Optimization of Cell-Free Protein Expression -- A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression -- One-Pot, Microscale Cell-Free Enzyme Expression and Screening -- Cell-Free Translation of Biofuels Enzymes -- Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems -- High Level Cell-Free Production of Membrane Proteins with Nanodiscs -- Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study -- Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis -- Cell-Free Protein Synthesis in Microfluidic 96-Well Plates -- Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis -- Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis -- In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate -- An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA -- In vitro Translation Based Protein Kinase Substrate Identification -- Preparation of Protein Arrays using Cell-Free Protein Expression -- Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System -- Cell-Free Membrane Protein Expression -- The PURE System for Protein Production -- A Cell-Free Protein Synthesis System from Insect Cells -- A Cell-Free Expression Platform for Production of Protein Microarrays Summary Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies Analysis eiwitsynthese protein synthesis eiwitten proteins Proteins and Enzymes Eiwitten en enzymen Notes Includes index Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed January 13, 2014) Subject Proteins -- Synthesis -- Laboratory manuals Life sciences. Gene expression. Metabolism. Physical sciences. Cells. Anatomy. Physiology. Subcellular Fractions Peptide Biosynthesis Biological Science Disciplines Gene Expression Cellular Structures Metabolism Natural Science Disciplines Biochemical Phenomena Genetic Phenomena Cells Chemical Phenomena Disciplines and Occupations Phenomena and Processes Anatomy Protein Biosynthesis Physiology Cell-Free System biological sciences. metabolism (biological concept) physical sciences. anatomy. physiology. Physiology Physical sciences Metabolism Life sciences Gene expression Cells Anatomy Proteins -- Synthesis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Alexandrov, Kirill, editor Johnston, Wayne A., editor LC no. 2013957131 ISBN 9781627037822 1627037829 1627037810 9781627037815

  Permalink