| Description |
xi, 322 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; v. 194 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 194
|
| Contents |
1. Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry / Jiang Wu and J. Throck Watson -- 2. Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods / George N. Saddic, Mary Beth Ebert and Shirish T. Dhume / [et al.] -- 3. Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) / Rolf Nuck -- 4. Separation of N-Glycans by HPLC / Martin Gohike -- 5. Enzymatic Sequence Analysis of N-Glycans / Christoph Kannicht and Anke Flechner -- 6. Immunological Detection of O-GlcNAc / Monika Rex-Mathes, Jurgen Koch and Sabine Werner / [et al.] -- 7. Analysis of O-Glycosylation / Juan J. Calvete and Libia Sanz -- 8. Characterization of Site-Specific Glycosylation / Katalin F. Medzihradszky -- 9. Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid / Shirish T. Dhume, Mary Beth Ebert and George N. Saddic / [et al.] -- 10. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa / Peter Gerold and Ralph T. Schwarz -- 11. Analysis of S-Acylation of Proteins / Michael Veit, Evgeni Ponimaskin and Michael F. G. Schmidt -- 12. Immunoblotting Methods for the Study of Protein Ubiquitination / Edward G. Mimnaugh and Leonard M. Neckers -- 13. Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins / Randy J. Arnold and James P. Reilly -- 14. Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry / Jun Qin and Xiaolong Zhang -- 15. Analysis of Tyrosine-O-Sulfation / Jens R. Bundgaard, Anders H. Johnsen and Jens F. Rehfeld -- 16. [alpha]-Amidated Peptides: Approaches for Analysis / Gregory P. Mueller and William J. Driscoll -- 17. [gamma]-Glutamate and [beta]-Hydroxyaspartate in Proteins / Francis J. Castellino, Victoria A. Ploplis and Li Zhang -- 18. Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides / Verne Schirch, Sonia Delle Fratte and Martino di Salvo -- 19. Lysine Hydroxylation and Crosslinking of Collagen / Mitsuo Yamauchi and Masashi Shiiba -- 20. Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications / Jens R. Bundgaard -- 21. 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern / Klemens Loster and Christoph Kannicht |
| Summary |
Post-translational modification of proteins : methods & protocols |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
Post-translational modification -- Laboratory manuals.
|
| Genre/Form |
Laboratory manuals.
|
| Author |
Kannicht, Christoph.
|
|
Humana Press.
|
| LC no. |
2001051864 |
| ISBN |
0896036782 alkaline paper |
|
