| Description |
1 online resource (xv, 588 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 |
|
Methods in molecular biology (Clifton, N.J.), 1940-6029
|
| Contents |
Flower development in Arabidopsis -- Flower development in the Solanaceae -- The ABC of flower development in monocots: the model of rice spikelet -- Model species to investigate the origin of flowers -- Hormones and flower development in Arabidopsis -- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors -- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development -- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice -- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize -- Genetic and phenotypic analyses of carpel development in Arabidopsis -- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis -- Floral induction systems for the study of Arabidopsis flower development -- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types -- Protoplast Isolation for Plant Single-Cell RNA-seq -- Plant Nuclei Isolation for Single-nucleus RNA Sequencing -- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis -- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution -- The GUS reporter system in flower development studies -- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species -- Gene expression analysis by quantitative real-time PCR for floral tissues -- Misexpression approaches for the manipulation of flower development -- Genomic approaches for the study of flower development in floriculture crops -- Multi-omics methods applied to flower development -- Peptidomics methods applied to the study of flower development -- Quantifying gene expression domains in plant shoot apical meristems -- A NanoLuc-based transactivation assay in plants -- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures |
| Summary |
This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 7, 2023) |
| Subject |
Flowers -- Development -- Laboratory manuals
|
|
Plant molecular biology -- Methodology
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Riechmann, José Luis, editor.
|
|
Ferrándiz, Cristina, editor
|
| ISBN |
9781071632994 |
|
107163299X |
|
