Description |
1 online resource (XI, 280 pages 62 illustrations, 54 illustrations in color.) |
Series |
Methods in molecular biology, 1940-6029 ; volume 2264 |
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Methods in molecular biology (Clifton, N.J.) ; v. 2264. 1064-3745
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Contents |
Methods of Development of Biparental Mapping Populations in Horticultural Crops -- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits -- Using Metabolomics to Assist Plant Breeding -- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications -- High Resolution Melting Analysis as a Tool for Plant Species Authentication -- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods -- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome -- Association Mapping in Plants -- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) -- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis -- RNA Interference (RNAi) in Tomato Crop Reseserch -- Protoplast Based Method for Genome Editing in Tetraploid Potato -- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria -- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant -- Assembly of TALEN and mTALE-Act for Plant Genome Engineering -- Genome Editing to Achieve the Crop Ideotype in Tomato -- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging -- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis |
Summary |
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field |
Bibliography |
Includes bibliographical references and index |
Subject |
Biomedical engineering.
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Agriculture.
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Transgenic plants.
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Plant Breeding -- methods
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Plants, Genetically Modified
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Agriculture
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Genome, Plant
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Crops, Agricultural -- genetics
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biomedical engineering.
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farming (activity or system)
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agriculture (discipline)
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Biomedical engineering
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Agriculture
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Crop improvement
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Crops -- Genetic engineering
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Plant breeding
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Tripodi, Pasquale., editor
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ISBN |
9781071612019 |
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1071612018 |
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9781071612026 |
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1071612026 |
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9781071612033 |
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1071612034 |
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