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Uniform Title Crop breeding (Tripoli)
Title Crop breeding : genetic improvement methods / edited by Pasquale Tripodi
Edition 1st ed. 2021
Published New York, NY : Springer US : Imprint : Humana, 2021

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Description 1 online resource (XI, 280 pages 62 illustrations, 54 illustrations in color.)
Series Methods in molecular biology, 1940-6029 ; volume 2264
Methods in molecular biology (Clifton, N.J.) ; v. 2264. 1064-3745
Contents Methods of Development of Biparental Mapping Populations in Horticultural Crops -- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits -- Using Metabolomics to Assist Plant Breeding -- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications -- High Resolution Melting Analysis as a Tool for Plant Species Authentication -- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods -- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome -- Association Mapping in Plants -- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) -- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis -- RNA Interference (RNAi) in Tomato Crop Reseserch -- Protoplast Based Method for Genome Editing in Tetraploid Potato -- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria -- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant -- Assembly of TALEN and mTALE-Act for Plant Genome Engineering -- Genome Editing to Achieve the Crop Ideotype in Tomato -- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging -- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis
Summary This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field
Bibliography Includes bibliographical references and index
Subject Biomedical engineering.
Agriculture.
Transgenic plants.
Plant Breeding -- methods
Plants, Genetically Modified
Agriculture
Genome, Plant
Crops, Agricultural -- genetics
biomedical engineering.
farming (activity or system)
agriculture (discipline)
Biomedical engineering
Agriculture
Crop improvement
Crops -- Genetic engineering
Plant breeding
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Tripodi, Pasquale., editor
ISBN 9781071612019
1071612018
9781071612026
1071612026
9781071612033
1071612034