| Description |
1 online resource (xi, 282 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1166 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1166. 1064-3745
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| Contents |
Introduction : plant cold acclimation and freezing tolerance / Dirk K. Hincha and Ellen Zuther -- Measuring freezing tolerance : survival and regrowth assays / Daniel Z. Skinner and Kimberly Garland-Campbell -- Measuring freezing tolerance : electrolyte leakage and chlorophyll fluorescence assays / Anja Thalhammer, Dirk K. Hincha, and Ellen Zuther -- Conducting field trials for frost tolerance breeding in cereals / Luigi Cattivelli -- A whole-plant screening test to identify genotypes with superior freezing tolerance / Annick Bertrand, Yves Castonguay, and Josée Bourassa -- Mapping of quantitative trait loci (QTL) associated with plant freezing tolerance and cold acclimation / Evelyne Téoulé and Carine Géry -- Common garden experiments to characterize cold acclimation responses in plants from different climatic regions / Andrey V. Malyshev, Hugh A.L. Henry, and Juergen Kreyling -- Identification of Arabidopsis mutants with altered freezing tolerance / Carlos Perea-Resa and Julio Salinas -- Infrared thermal analysis of plant freezing processes / Gilbert Neuner and Edith Kuprian -- Cryo-scanning electron microscopy to study the freezing behavior of plant tissues / Seizo Fujikawa and Keita Endoh -- Three-dimensional reconstruction of frozen and thawed plant tissues from microscopic images / David P. Livingston III and Tan D. Tuong -- Proteomic approaches to identify cold-regulated soluble proteins / Stefanie Döll, Rico Lippmann, and Hans-Peter Mock -- Proteomic approaches to identify cold-regulated plasma membrane proteins / Daisuke Takahashi [and four others] -- Profiling methods to identify cold-regulated primary metabolites using gas chromatography coupled to mass spectrometry / Frederik Dethloff [and eigt others] -- A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition / Hieu Sy Vu [and three others] -- Quantification of superoxide and hydrogen peroxide in leaves / Ilona Juszczak and Margarete Baier -- Estimating ice encasement tolerance of herbage plants / Bjarni E. Gudleifsson and Brynhildur Bjarnadottir -- Characterization of ice binding proteins from sea ice algae / Maddalena Bayer-Giraldi, EonSeon Jin, and Peter W. Wilson -- Isolation and characterization of ice-binding proteins from higher plants / Adam J. Middleton [and five others] |
| Summary |
Annotation Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 17, 2014) |
| Subject |
Acclimatization (Plants) -- Laboratory manuals
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Plants -- Frost resistance -- Laboratory manuals
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Plants -- Effect of cold on -- Laboratory manuals
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Acclimatization.
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Cold.
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Plants
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Plant Physiological Phenomena -- genetics
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Acclimatization
|
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Cold Temperature
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cold.
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vegetation.
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Cold
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Acclimatization
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Acclimatization (Plants)
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Plants -- Effect of cold on
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Plants -- Frost resistance
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Hincha, Dirk K., editor
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|
Zuther, Ellen, editor
|
| LC no. |
2014938067 |
| ISBN |
9781493908448 |
|
1493908448 |
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149390843X |
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9781493908431 |
|
