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Title Photorespiration : methods and protocols / edited by Alisdair R. Fernie, Hermann Bauwe, Andreas P.M. Weber
Published New York, NY : Humana Press, 2017

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Description 1 online resource (xiv, 274 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745
Methods in molecular biology (Clifton, N.J.), 1064-3745
Contents Estimation of Photorespiratory Fluxes by Gas Exchange -- Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling -- Measurement of Enzyme Activities -- In vitro Alkylation Methods for Assessing the Protein Redox State -- Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants -- In vitro Analysis of Metabolite Transport Proteins -- Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches -- Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration -- Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits -- Metabolic Engineering of Photorespiration -- 13CO2 Labelling and Mass Spectral Analysis of Photorespiration -- Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants -- Genome-scale Modeling of Photorespiratory Pathway Manipulation -- Kinetic Modelling of Photorespiration -- Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues -- Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana -- Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration
Summary This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed August 24, 2017)
Subject Plants -- Photorespiration -- Laboratory manuals
Plant physiology.
Photosynthesis -- physiology
Plant Stomata -- metabolism
Carbon Dioxide -- metabolism
Plant Physiological Phenomena
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Plant physiology
Plants -- Photorespiration
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Fernie, Alisdair R., editor.
Bauwe, Hermann, editor
Weber, Andreas P. M., editor
ISBN 9781493972258
1493972251