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Title Protein phosphorylation : a practical approach / edited by D.G. Hardie
Edition Second edition
Published London ; New York : Oxford University Press, 1999
Online access available from:
EBSCO eBook Academic Collection    View Resource Record  


Description 1 online resource (xxviii, 431 pages) : illustrations
Series The practical approach series ; 211
Practical approach series ; 211
Contents Study of protein phosphorylation in intact cells -- Analysis of signal transduction pathways using protein kinase inhibitors and activators -- Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors -- Analysis of signal transduction pathways using molecular biological manipulations -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates -- Phosphorylation site analysis by mass spectrometry -- Assay and purification of protein (serine/threonine) phosphatases -- Identification of substrates for protein-tyrosine phosphateases -- Assay and purification of protein-serine/threonine kinases -- Assay of protein kinases and phosphatases using specific peptide substrates -- Cloning and expression of cDNAs encoding protein kinase subunits -- Interaction cloning of protein kinase partners -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation -- Analysis of protein kinase interactions using the two-hybrid method -- Analysis of protein kinase interactions using biomolecular interaction analysis -- Analysis of protein kinase specificity using oriented peptide libraries -- Crystallization of protein kinases and phosphatases
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Phosphoprotein phosphatases -- Laboratory manuals.
Phosphoproteins -- Laboratory manuals.
Phosphorylation -- Laboratory manuals.
Protein kinases -- Laboratory manuals.
Protein Kinases.
Genre/Form Laboratory manuals.
Laboratory manuals.
Form Electronic book
Author Hardie, D. G. (D. Grahame)
ISBN 0585483930 (electronic bk.)
9780585483931 (electronic bk.)