Title Lipase and phospholipase protocols / edited by Mark Doolittle and Karen Reue

Published Totowa, N.J. : Humana Press, ©1999

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Description 1 online resource (xv, 362 pages) : illustrations Series Methods in molecular biology ; v. 109 Methods in molecular biology (Clifton, N.J.) ; v. 109 Contents Phospholipase A2 and phosphatidylinositol-specific phospholipase C assays by HPLC and TLC with fluorescent substrate / H.S. Hendrickson -- Fluorometric phospholipase assays based on polymerized liposome substrates / W. Cho [and others] -- Triglyceride lipase assays based on a novel fluorogenic alkyldiacyl glycerol substrate / A. Hermetter -- Purification and assay of mammalian group I and group IIa secretory phospholipase A2 / W. Cho [and others] -- Determination of plasmalogen-selective phospholipase A2 activity by radiochemical and fluorometric assay procedures / A.A. Farooqui [and others] -- Human plasma platelet-activating factor acetylhydrolase / D.M. Stafforini and L.W. Tjoelker -- Assays for pancreatic triglyceride lipase and colipase / M.E. Lowe -- Bile salt-activated lipase / C.S. Wang, A. Dashti and D. Downs -- Determining lipoprotein lipase and hepatic lipase activity using radiolabeled substrates / V. Briquet-Laugier, O. Ben-Zeev and M.H. Doolittle -- Lysosomal acid lipase. Assay and purification / M. Merkel [and others] -- Hormone-sensitive lipase and neutral cholesteryl ester lipase / C. Holm and T. Osterlund -- Lecithin-cholesterol acyltransferase. Assay of cholesterol esterification and phospholipase A2 activities / J.S. Parks, A.K. Gebre and J.W. Furbee -- Purification of lipases and phospholipases by heparin-sepharose chromatography / A.A. Farooqui and L.A. Horrocks -- Large-scale lipoprotein lipase purification from adipose tissue / A. Bensadoun, J. Hsu and B. Hughes -- Purification of rat hepatic lipase essentially free of apolipoprotein E and apolipoprotein B / A. Bensadoun [and others] -- High-level expression and purification of human hepatic lipase from mammalian cells / J.S. Hill -- High-level baculoviral expression of hormone-sensitive lipase / C. Holm and J.A. Contreras -- High-level baculoviral expression of lysosomal acid lipase / A.C. Tilkorn [and others] -- One-step purification and biochemical characterization of recombinant pancreatic lipases expressed in insect cells / S. Bezzine [and others] -- Modulation of the expression level of human acidic lipases by various signal peptides / S. Canaan [and others] -- Immunodetection of lipoprotein lipase: antibody production, immunoprecipitation, and western blotting techniques / M.H. Doolittle and O. Ben-Zeev -- Immunological characterization of digestive lipases / A. De Caro [and others] -- Determining lipase subunit structure by sucrose gradient centrifugation / O. Ben-Zeev and M.H. Doolittle -- Detecting ligands interacting with lipoprotein lipase / S. Pillarisetti -- -- -- Monolayer techniques for studying lipase kinetics / S. Ransac [and others] -- Efficient immobilization of phospholipase A2 / W. Cho and Z. Shen -- An introduction to Internet resources for the molecular and genetic analysis of the lipases / K. Reue -- Techniques for the measurement of lipoprotein lipase messenger RNA / G. Ranganathan and P.A. Kern -- In vitro transcription and translation of lipoprotein lipase / G. Ranganathan and P.A. Kern -- Induced lipase mutations in the mouse / C.F. Semenkovich Summary Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes Analysis triacylglycerol lipase fosfolipase a phospholipase a fosfolipase a1 phospholipase a1 fosfolipase a2 phospholipase a2 Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Lipase -- Laboratory manuals Phospholipases -- Laboratory manuals Phospholipases. Lipase Phospholipases Lipase. Phospholipases. Genre/Form Laboratory manuals. Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Doolittle, Mark Henry Reue, Karen ISBN 9781592595815 1592595812 128083692X 9781280836923 9786610836925 6610836922

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