Title Functional characterization of arabidopsis phosphatidylinositol monophosphate 5-kinase 2 in lateral root development, gravitropism and salt tolerance / Yu Mei

Published Dordrecht : Springer, [2014] ©2014

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Description 1 online resource : illustrations Series Springer theses Springer theses. Contents Supervisor�s Foreword -- Abstract -- Acknowledgments -- Contents -- About the Author -- 1 Review of Phosphatidylinositol Phosphate Kinase in Phosphatidylinositol Signaling Pathway -- Abstract -- 1.1emspBrief Introduction of Phosphatidylinositol Signaling Pathway -- 1.2emspPhosphatidylinositol Phosphate Kinase and Phosphatidylinositol Bisphosphate in Phosphatidylinositol Pathway -- 1.2.1 Classification of PIPKs -- 1.2.2 Substrates and Products of PIPKs -- 1.2.3 Regulation of PIPKs -- 1.2.4 Biological Functions of PIPKs 1.3emspResearch Progresses of PIPKs in Higher Plants1.3.1 PIPKs in Oryza Sativa -- 1.3.2 PIPK Family in Arabidopsis and Their Functions -- 1.4emspSummary and Prospective -- References -- 2 Structure and Expression Pattern Analysis of Arabidopsis PIP5K2 -- Abstract -- 2.1emspIntroduction -- 2.2emspMaterials and Methods -- 2.2.1 Plant Material -- 2.2.2 Strains and Plasmids -- 2.2.2.1 Strains -- 2.2.2.2 Plasmid Vectors -- 2.2.3 Chemicals and Reagents -- 2.2.4 Methods -- 2.2.4.1 Arabidopsis Growth Conditions 2.2.4.2 Agrobacterium-Mediated Arabidopsis Transformation2.2.4.3 RNA Extraction, RT-PCR, and Quantitative Real-Time RT-PCR Analysis -- 2.2.4.4 Promoter-GUS Fusion Studies and Histochemical Analysis of GUS Activity -- 2.2.4.5 Subcellular Localization Analysis -- 2.3emspResults -- 2.3.1 Structural Analysis of PIP5K2 Gene and Protein -- 2.3.2 Expression Pattern Analysis of PIP5K2 -- 2.3.3 PIP5K2 Is Mainly Localized on the Plasma Membrane -- 2.4emspDiscussion -- References 3 Arabidopsis PIP5K2 Is Involved in Lateral Root Development Through Regulating Auxin AccumulationAbstract -- 3.1emspIntroduction -- 3.2emspMaterials and Methods -- 3.2.1 Plant Materials -- 3.2.2 Strains and Plasmids -- 3.2.2.1 Strains -- 3.2.2.2 Plasmid Vectors -- 3.2.3 Chemicals and Reagents -- 3.2.4 Methods -- 3.2.4.1 Arabidopsis Growth Conditions -- 3.2.4.2 Agrobacterium-Mediated Arabidopsis Transformation -- 3.2.4.3 RNA Extraction and Quantitative Real-Time RT-PCR Analysis -- 3.2.4.4 Identification of T-DNA Insertion Knockout Mutant 3.2.4.5 Generation of PIP5K2 Transgenic Lines in Wild Type and pip5k2 Background3.2.4.6 Lateral Root Observation and Quantification -- 3.2.4.7 Detection of Auxin Accumulation and Distribution -- 3.2.4.8 Accession Numbers -- 3.3emspResults -- 3.3.1 Identification of PIP5K2 Knockout Mutant -- 3.3.2 Generation of PIP5K2 Transgenic Lines with Enhanced Expression or Transformation Rescue of PIP5K2 -- 3.3.3 pip5k2 Seedlings Have Reduced Lateral Root Formation, Which Can Be Rescued by Exogenous Auxin -- 3.3.4 Transformation Rescue of PIP5K2 Recovers the Phenotype of pip5k2 Summary The functional characterization of a key enzyme in the phosphatidylinositol (PI) signaling pathway in the model plant Arabidopsis thaliana is the focus of this thesis. Moreover, a particular focus is the exploration of the biological functions of Arabidopsis phosphatidylinositol monophosphate 5-kinase 2 (PIP5K2), which catalyzes the synthesis of phosphatidylinositol (4,5) bisphosphate, the precursor of two important second messengers (inositol 1,4,5-trisphosphate and diacylglycerol). Employing molecular and genetic approaches, the author isolates and characterizes the expression pattern, physiological functions and underlying mechanism of Arabidopsis PIP5K2. In doing so, he reveals that PIP5K2 is involved in regulating lateral root formation and root gravity response through modulating auxin accumulation and polar auxin transport, and also plays a critical part in salt tolerance. These findings shed new light on the crosstalk between PI signaling and auxin response, both of which fulfill crucial regulatory roles in plant development Analysis plantenfysiologie plant physiology plantenontwikkeling plant development plantenanatomie plant anatomy plantensamenstelling plant composition biochemie biochemistry Plant Physiology Plantenfysiologie Notes "Doctoral Thesis accepted by University of Chinese Academy of Sciences, China." Bibliography Includes bibliographical references Notes English Print version record In Springer eBooks Subject Phosphoinositides. Arabidopsis. Protein kinases. Cellular signal transduction. Biological Science Disciplines Plant Physiological Phenomena SCIENCE -- Life Sciences -- Biochemistry. Arabidopsis Cellular signal transduction Phosphoinositides Protein kinases biological sciences. biochemistry. Form Electronic book ISBN 9789401793735 9401793735

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