| Description |
1 online resource (xi, 238 pages) : illustrations |
| Series |
Methods in molecular medicine ; 28 |
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Methods in molecular medicine ; 28.
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| Contents |
Drug resistance : The clinical perspective / D.A. Anthoney and S.B. Kaye-Cell sensitivity assays : Clonogenic assay / J.A. Plumb-Cell sensitivity assays : The mtt assay / J.A. Plumb-Cell sensitivity assays : Detection of apoptotic cells in vitro using the tunel assay / N.A. Jones and C. Dive-Analysis of apoptosis in tissue sections / V. Save, P.J. Coates and P.A. Hall-Measurement of p-glycoprotein function / H.J. Broxterman-Measuring mdr-1 by quantitative rt-pcr / S.E. Bates [and others]-Microtiter plate technique for the measurement of glutathione in fresh and cryopreserved lymphoblasts using the enzyme recycling method / P.R. Kearns and A.G. Hall-Measurement of reduced glutathione using high-pressure liquid chromatography / L.A. Hogarth, C.M. Rabello and A.G. Hall-Topoisomerase i and ii activity assays / P. Pourquier [and others]-5-fluorouracil metabolizing enzymes / H.L. McLeod, L.H. Milne and S.J. Johnston-Measuring DNA adducts by immunoassay (elisa) / M.J. Tilby-Measuring drug-DNA adducts in individual cells / A.J. Frank-Measurement of drug-induced DNA interstrand crosslinking using the single-cell gel electrophoresis (comet) assay / V.J. Spanswick [and others]-Pcr analysis of microsatellite instability / G.L. Hirst-O (6)-alkylguanine-DNA alkyltransferase assay / A.J. Watson and G.P. Margison-Analysis of the p53 status of tumors : An overview of methods / J. Bergh-Bcl-2 family immunohistochemistry / L.R. Kelland and P.J. Beale-Genetic analysis of drug resistance by fluorescence in situ hybridization / W.N. Keith-Genetic analysis of drug resistance by reverse in situ hybridization / W.N. Keith |
| Summary |
In Cytotoxic Drug Resistance Mechanisms, leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms makes it possible to test established and laboratory-derived hypotheses with clinically derived tumor samples. State-of-the-art and readily reproducible, the methods presented here afford basic and clinical scientists a powerful complement of tools for investigating all the clinically relevant mechanisms used as markers of the biological response to anticancer chemotherapeutics today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Drug resistance in cancer cells -- Research -- Methodology
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Drug resistance in cancer cells.
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DNA damage.
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Genetics -- Technique.
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Drug Resistance, Neoplasm
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Antineoplastic Agents -- pharmacology
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DNA Damage
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Genetic Techniques
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MEDICAL -- Oncology.
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HEALTH & FITNESS -- Diseases -- Cancer.
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Genetics -- Technique
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DNA damage
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Drug resistance in cancer cells
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Résistance aux médicaments antinéoplasiques.
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Antinéoplasiques -- Pharmacologie.
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Lésions de l'ADN.
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Techniques génétiques.
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Cytotoxiciteit.
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Geneesmiddelenresistentie.
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Wetenschappelijke technieken.
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Résistance aux médicaments.
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Anticancéreux.
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ADN -- Altération.
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Cancer -- Résistance aux médicaments.
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Brown, Robert, 1956 January 8-
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Böger-Brown, Uta.
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| ISBN |
9781592596874 |
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1592596878 |
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