Title Chemical genomics : reviews and protocols / edited by Edward D. Zanders

Published Totowa, N.J. : Humana Press, [2005] ©2005

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Description 1 online resource (xi, 279 pages) : illustrations Series Methods in molecular biology ; v. 310 Methods in molecular biology (Clifton, N.J.) ; v. 310 Contents Introduction to chemical genomics / Paul R. Caron -- Chemistry for chemical genomics / Lutz Weber -- Computer-aided design of small molecules for chemical genomics / Philip M. Dean -- Design, synthesis, and screening of biomimetic ligands for affinity chromatography / Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe -- The role and application of in silico docking in chemical genomics research / Aldo Jongejan [and others] -- Synthesis of complex carbohydrates and glyconjugates: enzymatic synthesis of globotetraose using 1,3-N-acetylgalactosaminyltransferase LgtD from Haemophilus infuenzae strain Rd / Kang Ryu [and others] -- High-throughput cloning for proteomics research / Sharon A. Doyle Screening for the expression of soluble recombinant protein in Escherichia coli / Sharon A. Doyle -- High-throughput purification of hexahistidine-tagged proteins expressed in Escherichia coli / Michael B. Murphy and Sharon A. Doyle -- The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information / Tatsuya Sawasaki [and others] -- Advances in genome-wide protein expression using the wheat germ cell-free system / Yaeta Endo and Tatsuya Sawasaki -- Production of proteins for NMR studies using the wheat germ cell-free system / Toshiyuki Kohno -- Adenoviral expression of reporter proteins for high-throughput cell-based screening / Jon Hoyt and Randall W. King Summary Chemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets Bibliography Includes bibliographical references and index Notes Print version record Subject Genomics. Proteomics. Pharmacogenetics. Biochemical genetics. DNA microarrays. Combinatorial chemistry. Pharmaceutical technology. Biology. Genetics -- Technique. Life sciences. Physical sciences. Methodology. Proteins. Molecular probes. Ligands. Genomics -- methods -- Laboratory Manuals Combinatorial Chemistry Techniques -- Laboratory Manuals Ligands -- Laboratory Manuals Proteins -- chemical synthesis -- Laboratory Manuals Microarray Analysis Investigative Techniques Molecular Probe Techniques Genetics Laboratory Chemicals Publication Formats Amino Acids, Peptides, and Proteins Technology, Pharmaceutical Computational Biology Analytical, Diagnostic and Therapeutic Techniques and Equipment Specialty Uses of Chemicals Biology Genetic Techniques Microchip Analytical Procedures Publication Characteristics Chemicals and Drugs Chemical Actions and Uses Chemistry Techniques, Analytical Biological Science Disciplines Natural Science Disciplines Disciplines and Occupations Methods Genomics Protein Array Analysis Combinatorial Chemistry Techniques Proteins Molecular Probes Laboratory Manuals Protein Array Analysis -- methods -- Laboratory Manuals Ligands Proteomics Pharmacogenetics Molecular Biology biology. biological sciences. physical sciences. methodology. protein. SCIENCE -- Life Sciences -- Genetics & Genomics. Proteins Physical sciences Pharmaceutical technology Molecular probes Methodology Ligands Life sciences Genetics -- Technique Biology Biochemical genetics Combinatorial chemistry DNA microarrays Genomics Pharmacogenetics Proteomics Genomik Molekulargenetik Proteomanalyse Biology. Health & Biological Sciences. Genetics. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Zanders, Edward D. LC no. 2005013118 ISBN 9781592599486 1592599486

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