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Title Phagocytosis and phagosomes : methods and protocols / edited by Roberto Botelho
Published New York, NY : Humana Press, [2016]


Description 1 online resource (xii, 360 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1519
Methods in molecular biology (Clifton, N.J.) ; 1519. 1064-3745
Contents Phagocytosis : hungry, hungry cells / Matthew Gray and Roberto J. Botelho -- Analysis of human and mouse neutrophil phagocytosis by Flow Cytometry / Noah Fine, Oriyah Barzilay, and Michael Glogauer -- Quantitative efferocytosis assays / Amanda L. Evans, Jack W.D. Blackburn, Charles Yin, and Bryan Heit -- Quantifying phagocytosis by immunofluorescence and microscopy / Christopher H. Choy and Roberto J. Botelho -- Single cell analysis of phagocytosis, phagosome maturation, phagolysosomal leakage, and cell death following exposure of macrophages to silica particles / Gaurav N. Joshi, Renée M. Gilberti, and David A. Knecht -- Quantitative live-cell fluorescence microscopy during phagocytosis / Stella M. Lu, Sergio Grinstein, and Gregory D. Fairn -- Intracellular manipulation of phagosomal transport and maturation using magnetic tweezers / Shashank Shekhar, Vinod Subramaniam, and Johannes S. Kanger-- Quantitative immunofluorescence to study phagosome maturation / Roya M. Dayam and Roberto J. Botelho -- Using fluorescence resonance energy transfer-based biosensors to probe Rho GTPase activation during phagocytosis / Veronika Miskolci, Louis Hodgson, and Dianne Cox -- Analysis of LC3-associated phagocytosis and antigen presentation / Laure-Anne Ligeon, Susana Romao, and Christian Münz -- Quantitative spatiotemporal analysis of phagosome maturation in live cells / Laura Schnettger and Maximiliano G. Gutierrez -- Measuring phagosomal pH by fluorescence microscopy / Johnathan Canton and Sergio Grinstein -- Image-based analysis of phagocytosis : measuring engulfment and internalization / Nicholas D. Condon, Adam A. Wall, Jeremy C. Yeo, Nicholas A. Hamilton, and Jennifer L. Stow -- Fluorometric approaches to measuring reductive and oxidative events in phagosomes / Dale R. Balce and Robin M. Yates -- Simultaneous analysis of multiple lumenal parameters of individual phagosomes using high-content imaging / Samuel Cheung, Catherine Greene, and Robin M. Yates -- Isolation and western blotting of latex-bead phagosomes to track phagosome maturation / Anetta Härtlova, Julien Peltier, Orsolya Bilkei-Gorzo, and Matthias Trost -- Assessing the phagosome proteome by quantitative mass spectrometry / Julien Peltier, Anetta Härtlova, and Matthias Trost -- Dissecting phagocytic removal of apoptotic cells in Caenorhabditis elegans / Shiya Cheng, Kai Liu, Chonglin Yang, and Xiaochen Wang -- Measurement of Salmonella enterica internalization and vacuole lysis in epithelial cells / Jessica A. Klein, TuShun R. Powers, and Leigh A. Knodler -- Bacterial binding, phagocytosis, and killing : measurements using colony forming units / Kyle E. Novakowski, Dessi Loukov, Vikash Chawla, and Dawn M.E. Bowdish -- Filamentous bacteria as targets to study phagocytosis / Akriti Prashar, Sana I.S. Khan, and Mauricio R. Terebiznik -- Growing and handling of Mycobacterium tuberculosis for macrophage infection assays / Evgeniya V. Nazarova and David G. Russell -- Mycobacterium tuberculosis : readouts of bacterial fitness and the environment within the phagosome / Shumin Tan, Robin M. Yates, and David G. Russell -- Using flow cytometry to analyze cryptococcus infection of macrophages / Robert J. Evans, Kerstin Voelz, Simon A. Johnston, and Robin C. May
Summary This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time
Notes Includes index
Online resource; title from PDF title page (SpringerLink, viewed November 14, 2016)
Subject Phagocytosis -- Laboratory manuals
Microbiology (non-medical).
Science -- Life Sciences -- Biology -- Microbiology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Botelho, Roberto, 1974- editor.
ISBN 9781493965816