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Title Peroxynitrite detection in biological media : challenges and advances / editors: Serban Peteu, Sabine Szunerits, Mekki Bayachou
Published [Cambridge] : Royal Society of Chemistry, [2015]


Description 1 online resource
Series RSC detection science ; number 7
RSC detection science series ; no. 7.
Contents Cover; Peroxynitrite Detection in Biological Media Challenges and Advances; Preface; Dedication; Contents; Chapter 1 -- Peroxynitrite: The Basics; 1.1 History; 1.1.1 Before 1990; 1.1.2 1990 and Later; 1.2 Peroxynitrite In Vivo; 1.3 Challenges to the Detection of Peroxynitrite; 1.4 Nomenclature; Acknowledgements; References; Chapter 2 -- Quantifying Peroxynitrite: Bridging the Gap Between Chemistry, Biology and Immunology; 2.1 General Remarks on the Concept of "Redox Signaling"; 2.2 The Basic Chemical Properties of Peroxynitrite Compared with Other RNS/ROS as Signaling Molecules
2.3 The Complex Interplay of Peroxynitrite and Other RNS/ROS in Biological Systems2.4 Implications of Selecting a Model System for In Vitro, In Vivo and Ex Vivo Experiments; 2.4.1 Use of Biological Sample Materials; 2.4.2 Performing Cell Culture Studies; 2.4.3 Use of Scavengers, Pharmaceuticals or Enzymes to Alter Peroxynitrite Signaling Pathways; 2.5 General Challenges of Detecting RNS and ROS: The Complexity of the Task to Quantify Peroxynitrite in Biological Samples; 2.5.1 Overview of the Different Quantification Approaches
2.5.2 Parameters Impacting the Performance of ONOO− Quantification2.6 Conclusions; Acknowledgements; References; Chapter 3 -- Methods of Peroxynitrite Synthesis in the Context of the Development and Validation of Peroxynitrite Sensors; 3.1 Background; 3.2 Overview of Major Methods of Peroxynitrite Synthesis; 3.2.1 Peroxynitrite from the Reaction of Nitrite with Acidified Hydrogen Peroxide; 3.2.2 Synthesis of Peroxynitrite in a Two-Phase System Using Isoamyl Nitrite and H2O2; 3.2.3 The Reaction of H2O2 and Water Soluble Nitrites such as 2-Ethoxyethyl Nitrite in Basic Medium
3.2.4 The Reaction of Ozone with Azide Ions in the Presence of a Low Concentration of Alkali3.2.5 Other Methods of Peroxynitrite Synthesis; 3.2.6 Generation of Peroxynitrite from 3-(4-Morpholinyl) Sydnonimine Hydrochloride; 3.3 Advantages and Limitations of the Various Peroxynitrite Synthesis Routes; 3.3.1 Characterization of Peroxynitrite Sensors and Evaluation of Performance: Are Buffers Indifferent; 3.3.2 Advantages and Limitations of the Various Methods of Peroxynitrite Synthesis and Generation for Sensing Purposes; References
Chapter 4 -- Peroxynitrite-Sensitive Electrochemically Active Matrices4.1 Introduction: Challenges for the Formation of Selective and Sensitive Matrices for Peroxynitrite Detection; 4.2 Polymeric Films; 4.2.1 Metallophthalocyanine Films (MPCs); 4.2.2 Porphyrins; 4.2.3 Conducting Polymers; 4.3 Graphene-Based Matrices; 4.4 Conclusion and Perspectives; Acknowledgements; References; Chapter 5 -- Electrochemical Detection of Peroxynitrite in Biological Solutions: Challenges and Perspectives; 5.1 Challenges in the Detection of Peroxynitrite in Biological Conditions
Summary Peroxynitrite is a powerful oxidiser which can damage a wide array of molecules within cells, including DNA and proteins, leading to apoptosis, inflammation or cancer. Peroxynitrite detection and quantification provides critical information in understanding its biological implications. Attempts to investigate the behavior of peroxynitrite in vivo and in vitro have been hampered by the difficulty in detecting this highly reactive oxygen species. This book presents the current state of the art in this research field with contributions from scientific leaders in the field. The chapters make clear the associated challenges and development for selective and sensitive detection of peroxynitrite. This book is a timely addition to the literature, as the first in the field, dedicated to detecting this molecule in vivo. It will be welcomed by the community particularly medicinal and analytical chemists, developers of sensors and probes and analytical equipment manufacturers
Notes Includes index
Online resource; title from title details screen (Royal Society of Chemistry, viewed November 19, 2015)
Subject Peroxynitrite.
Nitrous acid.
Nitrogen compounds.
Inorganic compounds.
Peroxynitrous Acid
Reactive Nitrogen Species
Nitrous Acid
Nitrogen Compounds
Inorganic Chemicals
Chemicals and Drugs
Biosensing Techniques
nitrogen compounds.
Analytical chemistry.
SCIENCE -- Life Sciences -- Biochemistry.
Form Electronic book
Author Peteu, Serban, editor.
Szunerits, Sabine, editor.
Bayachou, Mekki, editor
Royal Society of Chemistry (Great Britain)
ISBN 9781782622352