Title Type 2 diabetes : methods and protocols / edited by Claire Stocker

Published New York : Humana Press, ©2009

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Description 1 online resource (x, 278 pages) : illustrations (some color) Series Springer protocols Methods in molecular biology, 1064-3745 ; 560 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 560 Contents 1. Selecting the "right" mouse model for metabolic syndrome and type 2 diabetes research / Edward H. Leiter -- 2. Nutritional models of type 2 diabetes mellitus / Beverly Sara Muhlhausler -- 3. The isolation and purification of rodent pancreatic islets of Langerhans / Jacqueline F.O ̓Dowd -- 4. The measurement of insulin secretion from isolated rodent islets of Langerhans / Anna L. Nolan and Jacqueline F.O ̓Dowd -- 5. The incubation and monitoring of cell viability in primary rat islets of Langerhans and pancreatic B-cell lines / Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell -- 6. In vitro culture of isolated islets of Langerhans : analysis of function / Anna L. Nolan -- 7. Single cell RT-PRC identification of genes expressed by human islet endocrine cells / Dany Muller, Peter M. Jones, and Shanta J. Persaud -- 8. Laser capture microdissection of human pancreatic B-cells and RNA preparation for gene expression profiling / Lorella Marselli [and others] -- 9. In vitro transdifferentiation of human hepatoma cells into pancreatic-like cells / Wan-chun Li -- 10. The measurement of GLUT4 translocation in 3T3-L1 adipocytes / Nicky Konstantopoulos and Juan Carlos Molero-Navajas -- 11. Pancreatic remodeling : beta cell apoptosis, proliferation and neogenesis and the measurement of beta cell mass and of individual beta cell size / Eduard Montanya and Noélia Téllez -- 12. Morphology of pancreatic islets : a time course of pre-diabetes in Zucker fatty rats / Petra Augstein and Eckhard Salzsieder -- 13. Fluorescent Immunohistochemistry and in situ hybridization analysis of pancreas / Xiuli Wang, Shundi Ge, and Gay M. Crooks -- 14. The measurement of insulin secretion using pancreas perfusion in the rodent / Edward T. Wargent -- 15. Hyperinsulinemic-euglycemic clamp to assess insulin sensitivity in vivo / Jason K. Kim -- 16. Gene expression analysis in diabetes research / Peter White and Klaus H. Kaestner -- 17. Gene expression mining in type 2 diabetes research / Donald R. Dunbar Summary Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease Analysis diabetes mellitus laboratoriummethoden laboratory methods protocollen protocols Cellular Biology Human Pathology Celbiologie Humane pathologie Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Type 2 diabetes -- Laboratory manuals Type 2 diabetes -- Susceptibility -- Research Type 2 diabetes -- Research Physiology. Type 2 diabetes. Pancreas. Insulin. Mice. Digestive organs. Proinsulin. Genetics -- Technique. Endocrine glands. Diabetes. Life sciences. Physical sciences. Anatomy. Endocrine glands -- Diseases. Muridae. Metabolism -- Disorders. Peptide hormones. Diseases. Hormones. Mammals. Vertebrates. Chordata. Physiology Diabetes Mellitus, Type 2 Gene Expression Profiling Laboratory Manuals Pancreas Insulin Islets of Langerhans Mice Digestive System Proinsulin Genetic Techniques Endocrine Glands Diabetes Mellitus Publication Formats Biological Science Disciplines Murinae Natural Science Disciplines Anatomy Publication Characteristics Pancreatic Hormones Endocrine System Endocrine System Diseases Glucose Metabolism Disorders Investigative Techniques Muridae Metabolic Diseases Peptide Hormones Analytical, Diagnostic and Therapeutic Techniques and Equipment Rodentia Disciplines and Occupations Disease Hormones Peptides Nutritional and Metabolic Diseases Mammals Hormones, Hormone Substitutes, and Hormone Antagonists Vertebrates Amino Acids, Peptides, and Proteins Chemicals and Drugs Chordata Animals Eukaryota Organisms physiology. biological sciences. physical sciences. anatomy. hormone. Mammalia (class) Vertebrata (subphylum) Chordata (phylum) Muridae (family) Vertebrates Proinsulin Physiology Physical sciences Peptide hormones Pancreas Muridae Mice Metabolism -- Disorders Mammals Life sciences Insulin Hormones Genetics -- Technique Endocrine glands -- Diseases Endocrine glands Diseases Digestive organs Diabetes Chordata Anatomy Type 2 diabetes Diabète de type 2 -- Cahiers de laboratoire. Diabetes mellitus Typ 2 Diabète -- Manuels de laboratoire. diabetes. laboratory diagnosis. diagnosis. methods. disease surveys. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Stocker, Claire. LC no. 2009922609 ISBN 9781597454483 1597454486

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