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Title Pancreatic cancer : methods and protocols / edited by Gloria H. Su
Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (xi, 352 pages) : illustrations
Series Methods in molecular medicine, 1543-1894 ; 103
Methods in molecular medicine ; 103. 1543-1894
Contents Identification and analysis of precursors to invasive pancreatic cancer / R.H. Hruban, R.E. Wilentz and A. Maitra -- Optimal molecular profiling of tissue and tissue components: Defining the best processing and microdissection methods for biomedical applications / G.S. Bova [and others] -- Immunohistochemistry and in situ hybridization in pancreatic neoplasia / R.E. Wilentz [and others] -- Practical methods for tissue microarray construction / H.L. Fedor and A.M. De Marzo -- Xenografting and harvesting human ductal pancreatic adenocarcinomas for DNA analysis / K. Walter, J. Eshleman and M. Goggins -- Culture and immortalization of pancreatic ductal epithelial cells / T. Lawson [and others] -- DNA methylation analysis in human cancer / C.D. Curtis and M. Goggins -- Digital single-nucleotide polymorphism analysis for allelic imbalance / H.W. Chang and M. Shih Ie -- Representational difference analysis as a tool in the search for new tumor suppressor genes / A. Hollestelle and M. Schutte -- Serial analyses of gene expression (sage) / J.L. Dai -- Oligonucleotide-directed microarray gene profiling of pancreatic adenocarcinoma / D.E. Misek [and others] -- Identification of differentially expressed proteins in pancreatic cancer using a global proteomic approach / C. Rosty and M. Goggins -- Detection of telomerase activity in patients with pancreatic cancer / K. Mizumoto and M. Tanaka -- Serological analysis of expression cdna libraries (serex): An immunoscreening technique for identifying immunogenic tumor antigens / Y.T. Chen, A.O. Gure and M.J. Scanlan -- Modeling pancreatic cancer in animals to address specific hypotheses / P.J. Grippo and E.P. Sandgren -- Strategies for the use of site-specific recombinases in genome engineering / J.R. Jones, K.D. Shelton and M.A. Magnuson -- Primary explant cultures of adult and embryonic pancreas / F. Esni [and others] -- Zebrafish as a model for pancreatic cancer research / N.S. Yee and M. Pack -- Development of a cytokine-modified allogeneic whole cell pancreatic cancer vaccine / D. Laheru [and others] -- Overview of linkage analysis: Application to pancreatic cancer / A.P. Klein
Summary In the last decade, major progress has been made in identifying the critical oncogenes and tumor-suppressor genes that can cause pancreatic cancer. In Pancreatic Cancer: Methods and Protocols, leading clinicians and scientists from prestigious research institutes and cancer centers present in readily reproducible format the classical techniques that have contributed to these advances in pancreatic research and introduce new strategies that should produce future breakthroughs across the field of cancer biology. The authors provide a broad range of methods for the molecular, biochemical, pathological, and statistical analyses of both sporadic and familial pancreatic cancers, methods that can be applied not only to basic, but also to translational pancreatic research. Topics covered include in vitro cell cultures, in vivo mouse models, protein studies, mutation analysis, and treatment development. The protocols follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pancreatic Cancer: Methods and Protocols offers those interested in either basic or translational research an ambitious collection of essential yet novel methods for pancreatic cancer research, methods that can also readily be applied broadly throughout cancer research
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer e-books
Subject Pancreas -- Cancer -- Research -- Methodology
Pancreas -- Cancer -- Molecular aspects
Genetics -- Technique.
Pancreatic Neoplasms -- genetics
Genetic Techniques
Pancreatic Neoplasms -- therapy
MEDICAL -- Oncology.
HEALTH & FITNESS -- Diseases -- Cancer.
Genetics -- Technique
Pancreas -- Cancer -- Molecular aspects
Pancreas -- Cancer -- Research -- Methodology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Su, Gloria H.
ISBN 1588291073
9781588291073
9781592597802
1592597807
1280360216
9781280360213
9786610360215
6610360219