| Description |
1 online resource (xii, 337 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1882 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1882. 1064-3745
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| Contents |
Subtyping of IPMN / Toru Furukawa -- Clinical management and surgical decision-making of IPMN of the pancreas / Masao Tanaka -- Develop nomogram to predict malignancy of intraductal papillary mucinous neoplasm / Yongkang Kim and Taesung Park -- Generation of induced pluripotent stem cell-like lines from human pancreatic ductal adenocarcinoma / Jungsun Kim and Kenneth S. Zaret -- Lineage tracing of primary human pancreatic acinar and ductal cells for studying acinar-to-ductal metaplasia / Jun Liu and Pei Wang -- Spheroid culture of human pancreatic ductal cells to reconstitute development of pancreatic intraepithelial neoplasia / James J. Lee and Seung K. Kim -- Pancreatic microtumors : a novel 3D ex vivo testing platform / Mackenzie Lee Goodwin, Sumi Kanthraj Urs, and Diane M. Simeone -- 3D models of pancreatic ductal adenocarcinoma via tissue engineering / Claudio Ricci and Serena Danti -- Generation and culture of human pancreatic ductal adenocarcinoma organoids from resected tumor specimens / Lindsey A. Baker, Herve Tiriac, and David A. Tuveson -- Generation and culture of tumor and metastatic organoids from murine models of pancreatic ductal adenocarcinoma / Lindsey A. Baker and David A. Tuveson -- In vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer : a culture system based on collagen gel embedding / Takashi Okumura, Kenoki Ohuchida, and Masafumi Nakamura -- Osteoclast differentiation assay / Jingxuan Yang, Xiaohong Bi, and Min Li -- Primary cultures for pancreatic stellate cells (PSCs) / Lei Tian, Zipeng Lu, and Yi Miao -- Cytokine profiling and orthotopic xenografing of pancreatic stellate cells / Dong Qian, Lei Tian, Zipeng Lu, and Yi Miao -- Quantitative method to track proteolytic invasion in 3D collagen / Kazumi Ebine, Christina R. Chow, and Hidayatullah G. Munshi -- Detection and quantification of macropinosomes in pancreatic tumors / Szu-Wei Lee, Basheer Alas, and Cosimo Commisso -- Evaluating stress granules in pancreatic cancer in vitro and in vivo / Edward Sim, Elena Irollo, and Elda Grabocka -- Methods for monitoring macroautophagy in pancreatic cancer cells / Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, and Ciro Isidoro -- Measurement of reactive oxygen species by fluorescent probes in pancreatic cancer cells / Yongde Luo, Dan Wang, James L. Abbruzzese, and Weiqin Lu -- Evaluating the metabolic alterations in pancreatic cancer / Aneesha Dasgupta, Surendra K. Shukla, Venugopal Gunda, Ryan J. King, and Pankaj K. Singh -- Isolation of extracellular vesicles for cancer diagnosis and functional studies / Alex W. Brenner, Gloria H. Su, and Fatemeh Momen-Heravi -- Evaluation of post-transcriptional gene regulation in pancreatic cancer cells : studying RNA binding proteins and their mRNA targets / Aditi Jain, Samantha Z. Brown, Henry L. Thomsett, Eric Londin, and Jonathan R. Brody -- Laser capture microdissection on frozen sections for extraction of high-quality nucleic acids / H. Carlo Maurer and Kenneth P. Olive -- Statistical methods for identifying biomarkers from miRNA profiles of cancers / Junghyun Namkung -- Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice / Lu Zhuang, Xianbao Zhan, Yan Bi, and Baoan Ji -- Pancreatic acinar-to-ductal metaplasia and pancreatic cancer / Liang Wang, Dacheng Xie, and Daoyan Wei -- Orthotopic pancreatic tumor mouse models of liver metastasis / George Zhang and Yi-Chieh Nancy Du -- Molecular and physiological evaluation of pancreatic cancer-induced cachexia / Surendra K. Shukla, Aneesha Dasgupta, Scott E. Mulder, and Pankaj K. Singh |
| Summary |
This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have showed to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways to improving patient care for pancreatic cancer |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Pancreas -- Cancer
|
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Genetics -- Technique.
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Pancreatic Neoplasms -- genetics
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Genetic Techniques
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Pancreatic Neoplasms -- therapy
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Pancreatic Neoplasms
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Oncology.
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Medical -- Oncology.
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Genetics -- Technique
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Pancreas -- Cancer
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| Genre/Form |
Electronic books
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| Form |
Electronic book
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Author |
Su, Gloria H., editor
|
| ISBN |
9781493988792 |
|
1493988794 |
|
