| Description |
1 online resource (xii, 389 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 980 |
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Methods in molecular biology (Clifton, N.J.) ; v. 980. 1064-3745
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| Contents |
Identification and analysis of precursors to invasive pancreatic cancer / Hanno Matthaei, Marco Dal Molin, and Anirban Maitra -- Tissue microarrays : construction and use / Helen Remotti -- Immunohistochemistry of pancreatic neoplasia / Sukhwinder Kaur [and others] -- Method for conducting highly sensitive MicroRNA in situ hybridization and immunohistochemical analysis in pancreatic cancer / Lorenzo F. Sempere and Murray Korc -- Optimal molecular profiling of tissue and tissue components : defining the best processing and microdissection methods for biomedical applications / Jaime Rodriguez-Canales -- Considerations for sequencing analyses of pancreatic cancer progression and metastasis / Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue -- DNA methylation analysis in human cancer / Eileen O'Sullivan and Michael Goggins -- Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients / Michael J. Baine, Kavita Mallya, and Surinder K. Batra -- Development of a cytokine-modified allogeneic whole cell pancreatic cancer vaccine / Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee -- Prevention and genetics of pancreatic cancer : a programmatic approach / Aimee L. Lucas [and others] -- Development of orthotopic pancreatic tumor mouse models / Wanglong Qiu and Gloria H. Su -- Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems / Emman Mascarinas, Guido Eibl, and Paul J. Grippo -- Quantification of murine pancreatic tumors by high-resolution ultrasound / Stephen A. Sastra and Kenneth P. Olive -- Microdissection and culture of murine pancreatic ductal epithelial cells / Yuliya Pylayeva-Gupta, Kyoung Eun Lee, and Dafna Bar-Sagi -- Sphere-forming assays for assessment of benign and malignant pancreatic stem cells / Yue J. Wang [and others] -- Analysis of transplanted bone marrow-derived cells in chronic pancreatitis / Christoph B. Westphalen, Frederic Marrache, and Timothy C. Wang -- Analysis of the potential for pancreatic cancer metastasis in vitro and in vivo / Chen Huang and Keping Xie -- Use of fluorescent probes in the study of reactive oxygen species in pancreatic cancer cells / Richard D. Dinnen, Yuehua Mao, and Robert L. Fine -- Analysis of tumor-associated mucin glycotopes by western transfer methods / Vinayaga S. Gnanapragassam, Maneesh Jain, and Surinder K. Batra -- Evaluating tumor angiogenesis / Minji K. Uh, Jessica Kandel, and Jan Kitajewski -- Pooled shRNA screenings : experimental approach / Ruth Rodriguez-Barrueco, Netonia Marshall, and Jose M. Silva -- Pooled ShRNA screenings : computational analysis / Jiyang Yu [and others] |
| Summary |
Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls |
|
Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer. |
| Analysis |
biomedische wetenschappen |
|
biomedicine |
|
neoplasms |
|
humane genetica |
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human genetics |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Pancreas -- Cancer -- Research -- Methodology
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Pancreas -- Cancer -- Molecular aspects
|
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Genetics -- Technique.
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Pancreatic Neoplasms -- genetics
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Genetic Techniques
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Pancreatic Neoplasms -- therapy
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Genetics -- Technique
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Pancreas -- Cancer -- Molecular aspects
|
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Pancreas -- Cancer -- Research -- Methodology
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Su, Gloria H.
|
| ISBN |
9781627032872 |
|
1627032878 |
|
