Title Pain research : methods and protocols / edited by David Z. Luo

Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xi, 303 pages) : illustrations Series Methods in molecular medicine, 1543-1894 ; 99 Methods in molecular medicine ; 99. 1543-1894 Contents Mechanistic dissection of pain: from DNA to animal models / Z. David Luo -- Assessment of acute thermal nociception in laboratory Animals / Jeffrey W. Allen and Tony L. Yaksh -- Tissue injury models of persistent nociception in rats / Jeffrey W. Allen and Tony L. Yaksh -- Segmental spinal nerve ligation model of neuropathic pain / Jin Mo Chung, Hee Kee Kim, and Kyungsoon Chung -- Partial sciatic nerve transection / Thies Lindenlaub and Claudia Sommer -- Modeling diabetic sensory neuropathy in rats / Nigel A. Calcutt -- Sciatic inflammatory neuropathy (SIN) in the rat : surgical procedures, induction of inflammation, and behavioral testing / Erin D. Milligan, Steven F. Marier, and Linda R. Watkins -- Loop dialysis catheter : a technology for chronic spinal dialysis in a freely moving rat / Martin Marsala -- Intrathecal catheterization and drug delivery in the rat / Shelle A. Malkmus and Tony L. Yaksh Trigeminal neuronal recording in animal models of orofacial pain / Koichi Iwata, Yuji Masuda, and Ke Ren -- In vivo electrophysiology of dorsal horn neurons / Louise C. Stanfa and Anthony H. Dickenson -- Single fiber recording : in vivo and in vitro preparations / Maria Schafers and David Cain -- Anatomical identification of neurons responsive to nociceptive stimuli / Luc Jasmin and Peter T. Ohara -- Isolation and culture of sensory neurons from the dorsal root ganglia of embryonic or adult rats / Thomas H. Burkey, Cynthia M. Hingtgen, and Michael R. Vasko -- Primary cultures of neonatal rat spinal cord / Virginia S. Seybold and Lia G. Abrahams -- Single-cell laser capture microdissection and rna amplification -- Fredrik Kamme [and others] -- Functional genomic analysis in pain research using hybridization arrays / Stephen J. Walker [and others] -- Generation of transgenic mice / Guoping Feng, Jing Lu, and Jimmy Gross -- Knockout mouse models in pain research / Andree Dierich and Brigitte Kieffer Summary Despite significant advances in molecular biology techniques and in our understanding of the physiology and the behavioral pharmacology of pain transduction, effective, target-specific therapeutic agents for chronic pain are still lacking. In Pain Research: Methods and Protocols, leading researchers who have first-hand experience describe, in step-by-step detail, diverse and novel techniques for dissecting the molecular mechanisms of pain transduction. These readily reproducible methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard, cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Versatile and easy-to-use, Pain Research: Methods and Protocols offers today's pain researchers in academic and pharmaceutical laboratories powerful tools to unravel the cellular and molecular complexity of pain transduction and set the stage for the next generation of pain medications Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Pain -- Animal models -- Laboratory manuals Animal experimentation. Mice. Rats. Pain -- physiopathology Animal Experimentation Mice Models, Animal Neural Pathways -- physiopathology Rats Signal Transduction -- physiology HEALTH & FITNESS -- Pain Management. MEDICAL -- Pain Medicine. Rats Mice Animal experimentation Pain -- Animal models Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Luo, Z. David. ISBN 9781592597703 159259770X 1588291030 9781588291035 1280358262 9781280358265 9786610358267 6610358265

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