| Description |
447 pages : illustrations ; 26 cm |
| Contents |
Includes index |
| Summary |
This book addresses the problem of cross-fertilization of methods from one lab to another, from one discipline to another, by allowing experts in the techniques free rein to describe the methods in detail, including specificatin of the source of reagents, pitfalls likely to be encountered, and other subtleties of the trade. This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems used most frequently, including all the three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. In section III, a large number of analytic techniques are presented for quantitation of both the radicals and their scavengers. In Section IV, cellular phenomena are described, including both generating systems and detection systems, as well as methods of enhancing cellular scavenger levels. Section V covers a number of miscellaneous techniques that are frequently cited in the literature |
| Notes |
Editor: Robert A.Greenwald.Includes bibliographical references |
| Bibliography |
Includes bibliographies and index |
|
Includes index |
| Subject |
Active oxygen -- Research -- Technique -- Handbooks, manuals, etc.
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Radicals (Chemistry) -- Research -- Technique -- Handbooks, manuals, etc.
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Superoxide dismutase -- Research -- Technique -- Handbooks, manuals, etc.
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Superoxides -- Research -- Technique -- Handbooks, manuals, etc.
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Active oxygen -- Research -- Technique -- Handbooks, manuals, etc.
|
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Description of technique
|
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Free radicals (Chemistry)
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Oxygen -- Analysis.
|
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Oxygen.
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Radicals (Chemistry) -- Research -- Technique -- Handbooks, manuals, etc.
|
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Radicals (Chemistry) -- Research -- Handbooks, manuals, etc.
|
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Research.
|
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Sampling and analysis
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Superoxide dismutase -- Research -- Technique -- Handbooks, manuals, etc.
|
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Superoxides -- Research -- Technique -- Handbooks, manuals, etc.
|
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Free Radicals.
|
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Oxygen -- analysis.
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Oxygen -- isolation & purification.
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| Genre/Form |
Handbooks and manuals.
|
| Author |
Greenwald, Robert A., 1943-
|
| LC no. |
85005277 |
| ISBN |
0849329361 |
|
