Title Bone research protocols / edited by Miep H. Helfrich, Stuart H. Ralston

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xiv, 448 pages) : illustrations Series Methods in molecular medicine ; 80 Methods in molecular medicine ; 80. 1543-1894 Contents Human osteoblast culture / J.A. Gallagher -- Osteoblast isolation from murine calvariae and long bones / A. Bakker and J. Klein-Nulend -- Mineralizing fibroblast-colony-forming assays / A. Scutt [and others] -- Osteocyte isolation and culture / P.J. Nijweide [and others] -- Isolated osteoclast cultures / A. Hoebertz and T.R. Arnett -- Primary isolation and culture of chicken osteoclasts / P. Collin-Osdoby, F. Anderson and P. Osdoby -- Isolation and purification of rabbit osteoclasts / F.P. Coxon [and others] -- Generating human osteoclasts from peripheral blood / A. Sabokbar and N.S. Athanasou -- Generating human osteoclasts in vitro from bone marrow and peripheral blood / A.M. Flanagan and H.M. Massey -- Generating murine osteoclasts from bone marrow / N. Takahashi [and others] -- Osteoclast formation in the mouse coculture assay / R.J. van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / P. Collin-Osdoby [and others] -- Analysis of osteoclast function in mouse calvarial cultures / M.J. Marshall and M.N. Rowlands -- Assessing bone formation using mouse calvarial organ cultures / I.R. Garrett -- In situ hybridization and in situ reverse transcription polymerase chain reaction in human bone sections / A.P. Mee and J.A. Hoyland -- Techniques for the study of apoptosis in bone / B.S. Noble and H.Y. Stevens -- Protein localization in wax-embedded and frozen sections of bone using immunohistochemistry / S. Bord -- Detection of noncollagenous bone proteins in methylmethacrylate-embedded human bone sections / J.P. van Leeuwen and P. Derkx -- Fluorescence imaging of bone-resorbing osteoclasts by confocal microscopy / S.A. Nesbitt and M.A. Horton -- Bone histomorphometry / S. Vedi and J. Compston -- Transmission electron microscopy of bone / V. Everts, A. Niehof and W. Beertsen -- Scanning electron microscopy of bone / D. Marshall, M.H. Helfrich and R.M. Aspden -- Bone measurements by peripheral quantitative computed tomography in rodents / J.A. Gasser -- Studies of local bone remodeling. The calvarial injection assay / R.J. van 't Hof, C.E. Clarkin and K.J. Armour -- Inflammation-induced osteoporosis. The IMO model / K.J. Armour and K.E. Armour -- Ovariectomy and estrogen replacement in rodents / J.W. Chow -- Mechanical testing of bone ex vivo / R.M. Aspden -- Bone cell responses to fluid flow / H.Y. Stevens and J.A. Frangos -- Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models / A.A. Pitsillides [and others] -- Extraction of nucleic acids from bone / T.L. Stewart and V. Mann -- Analysis of gene expression in bone by quantitative RT-PCR / D. Ireland Summary The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism Bibliography Includes bibliographical references and index Notes English Print version record Subject Bones -- Cultures and culture media -- Laboratory manuals Bone cells -- Laboratory manuals Histology -- Technique. Osteoclasts. Bone and Bones -- cytology Cell Culture Techniques Histological Techniques Osteoblasts Osteoclasts MEDICAL -- Physiology. SCIENCE -- Life Sciences -- Human Anatomy & Physiology. Osteoclasts Histology -- Technique Bone cells Bot (anatomie) Wetenschappelijke technieken. Gidsen (vorm) Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Helfrich, Miep H. Ralston, Stuart. ISBN 9781592593668 1592593666 1280842458 9781280842450 9786610842452 6610842450

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