Title Cardiac cell and gene transfer : principles, protocols, and applications / edited by Joseph M. Metzger

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xi, 253 pages) : illustrations Series Methods in molecular biology ; v. 219 Methods in molecular biology (Clifton, N.J.) ; v. 219 Contents pt. 1. Vectors for cardiac gene transfer: production, purification, and applications -- Adenoviral vectors: production and purification / Faris P. Albayya -- Gutted adenoviral vectors for gene transfer to muscle /Jeannine M. Scott -- Dual vector expansion of the recombinant AAV packaging capacity / Dongsheng Duan -- Lentivirus vector-mediated gene transfer to cardiomyocytes / Tsuyoshi Sakoda -- pt. 2. Cell transfer to myocardium: protocols and applications -- Cell therapy in the heart: cell production, transplantation, and applications / Kevin S. Cahill -- Cardiac cell transplantation: protocols and applications / Steven M. White -- Cell grafting for cardiac repair / Hans Reinecke -- pt. 3. Vectors for cardiac gene transfer: in vitro and in vivo assays and approaches -- Suppressor tRNAs: protocols and applications for cardiac gene transfer / Massimo Buvoli -- Antisense oligonucleotides: design, construction, and applications to cardiac allograft transfer / Leora B. Balsam -- Theoretical and technical considerations for gene transfer into vascularized cardiac transplants / Guanyi Lu -- Isolation, culture, and gene transfer of adult canine cardiac myocytes / Jennifer C. Hirsch -- Myofilament protein phosphorylation by PKC in genetically engineered adult cardiac myocytes / Margaret V. Westfall -- pt. 4. In vivo cardiac gene transfer: protocols, applications, and physiological assessment -- Embryonic and neonatal cardiac gene transfer in vivo / Geir Christensen -- Efficient viral gene transfer to rodent hearts in vivo / Federica del Monte -- Direct gene transfer to the adult rodent myocardium in vivo / Michael L. Szatkowski -- Adenovirus-mediated gene transfer to cardiac myocytes in vivo using catheter-based procedures / Damien Logeart -- Coronary perfusion cocktails for in vivo gene transfer / Stephan E. Lehnart -- Modification of in vivo cardiac performance by intracoronary gene transfer of B-adrenergic receptor signaling components / Hendrik T. Tevaearai -- Protocols for hemodynamic assessment of transgenic mice in vivo / Dimitrios Georgakopoulos Summary Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients Bibliography Includes bibliographical references and index Notes Print version record Subject Heart -- Diseases -- Gene therapy Genetic transformation. Heart cells. Myoblast transfer therapy. Gene therapy. Genetic Therapy Heart Diseases -- therapy Myoblasts -- cytology Myocytes, Cardiac -- cytology Transformation, Genetic HEALTH & FITNESS -- Diseases -- Heart. MEDICAL -- Cardiology. Gene therapy Genetic transformation Heart cells Myoblast transfer therapy Hartcellen. Gentherapie. Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Metzger, Joseph Mark, 1957- ISBN 9781592593507 0896039943 9780896039940 159259350X

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