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Title Mycobacteria protocols / edited by Tanya Parish and N.G. Stoker
Published Totowa, N.J. : Humana Press, [1998]
©1998

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Location Call no. Vol. Availability
 W'PONDS  579.374 Par/Mpr  AVAILABLE
Description xiv, 472 pages : illustrations ; 24 cm
Series Methods in molecular biology ; 101
Methods in molecular biology (Clifton, N.J.) ; v. 101
Contents Machine derived contents note: Mycobacteria: Bugs and Bugbears -- Tanya Parish and Neil G. Stoker -- Mycobacteria: General Culture Methodology and Safety Considerations -- Bryan W. Allen -- Isolation of Genomic DNA from Mycobacteria -- John T. Belisle and Michael G. Sonnenberg -- Nucleic-Acid Extraction: Plasmid/Cosmid DNA -- Bonnie B. Plikaytis and C. Harold King -- Pulsed Field Gel Electrophoresis for Mycobacteria -- Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and Stewart T. Cole -- Extraction of RNA from Mycobacteria -- Eshwar Mahenthiralingam -- Preparation of Cell-Free Extracts from Mycobacteria -- Tanya Parish and Paul R. Wheeler -- Preparation of Cell-Wall Fractions from Mycobacteria -- Gurdyal S. Besra -- Using MycDB on the World-Wide Web -- Staffan Bergh and Stewart T. Cole -- Conjugating DNA into Mycobacteria -- Julie-Ann Gavigan and Carlos Martin -- Electroporation of Mycobacteria -- Tanya Parish and Neil G. Stoker -- Mycobacteriophages -- Gary J. Sarkis and Graham F. Hatfull -- Chemical Mutagenesis of Mycobacteria -- Lucy A. Brooks -- Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System -- Esther Přez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel, and Carlos Martin -- Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement -- Robert N. Husson -- Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker -- Peter Sander and Erik C. Bṯtger -- Gene Replacement in Mycobacterium intracellulare -- Britt-Inger Marklund and Richard W. Stokes -- Gene Replacement in Mycobacterium bovis BCG -- Elizabeth Norman -- The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria -- Stephen Gordon, Gavin Chung, and Peter Andrew -- Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria -- Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, and V. Deretic -- Expression of Genes in Mycobacteria -- Peadar - Gaora -- Secretion of Mammalian Proteins from Mycobacteria -- Peter J. Murray and Richard A. Young -- Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR -- Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan -- Analysis of Mycobacterial Differential Gene Expression by RAP-PCR -- Joseph A. Mangan and Philip D. Butcher -- Mycobacterial Speciation -- Francis A. Drobniewski and Anne H. C. Uttley -- Species Identification of Mycobacteria Using rDNA Sequencing -- Philip Kirschner and Erik C. Bṯtger -- Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction -- Stuart M. Wilson -- Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping -- Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, and Jan D. A. van Embden -- Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis -- Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden -- Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism) -- Amalio Telenti, Nadine Honor,̌ and Stewart T. Cole -- The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria -- Paul F. Riska and William R. Jacobs, Jr -- Index
Notes Formerly CIP. Uk
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press
Subject Bacterial genetics -- Laboratory manuals.
Mycobacteria -- Laboratory manuals.
Mycobacteria.
Mycobacterium -- genetics.
DNA -- physiology.
Molecular Biology -- methods.
Mycobacterium -- growth & development.
DNA -- physiology.
Molecular Biology -- methods.
Mycobacterium -- genetics.
Mycobacterium -- growth & development.
Author Parish, Tanya.
Stoker, Neil G.
Humana Press.
LC no. 98022177
ISBN 0896034712 (alk. paper)