Title Mycobacterium tuberculosis protocols / edited by Tanya Parish and Neil G. Stoker

Published Totowa, NJ : Humana Press, ©2001

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Description 1 online resource (xiv, 403 pages) : illustrations Series Methods in molecular medicine ; 54 Methods in molecular medicine ; 54. 1543-1894 Contents Plasmid vectors / N. Casali and S. Ehrt -- Isolation of DNA from Mycobacterium tubercolosis / P.D. Helden [and others] -- Extraction of RNA from intracellular Mycobacterium tuberculosis / I.M. Monahan, J.A. Mangan and P.D. Butcher -- Transposon mutagenesis in mycobacteria using conditionally replicating mycobacteriophages / S.S. Bardarov, S.S. Bardarov and W.R. Jacobs -- Gene replacement and transposon delivery using the negative selection marker sac B / M. Jackson [and others] -- Gene replacement using pretreated DNA / B.G. Gordhan and T. Parish -- Gene replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG using rpsl as a dominant negative selectable marker / P. Sander, B. Springer and E.C. Böttger -- Transcription start-site mapping / F. Movahedzadeh, J.A. Gonzalez-Y-Merchand and R.A. Cox -- Fluorescence and Brightfield cytology of live M. tuberculosis cells / R.P. Cooney, N.J. Garton and M.R. Barer -- Phage replication technology for diagnosis and drug susceptibility testing / R. McNerney -- Detection of mutations in Mycobacterium tuberculosis by a dot blot hybridization strategy / T.C. Victor and P.D. Helden -- Restriction fragment length polymorphism typing of mycobacteria / D. Soolingen, P.E.W. Haas and K. Kremer -- Preparation of culture filtrate proteins from Mycobacterium tuberculosis / I. Rosenkrands and P. Andersen -- Analysis of the capsule of Mycobacterium tuberculosis / M. Daffé and M.-A. Lanée -- Analysis of the lipids of Mycobacterium tuberculosis / R.A. Slayden and C.E. Barry -- In vitro model of hypoxically induced nonreplicating persistence of Mycobacterium tuberculosis / L.G. Wayne -- 17 macrophage virulence assays / P.T. Lukey and E.U. Hooker -- Analysis of mycobacterium -infected macrophages by immunoelectron microscopy and cell fractionation / W. Beatty and D.G. Russell -- Real time PCR using molecular beacons / R. Manganelli, S. Tyagi and I. Smith -- Electronic access to Mycobacterium tuberculosis sequence data / J. Parkhill -- Proteomics / J.C. Betts and M.A. Smith -- Functional genomics of Mycobacterium tuberculosis using DNA microarrays / M. Wilson [and others] -- Storage of mycobacterial strains / K. Kremer, T. Laan and D. Soolingens -- Safety in the laboratory / H. Alderton and D. Smith Summary With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Tuberculosis -- Molecular aspects -- Laboratory manuals Bacterial genetics -- Laboratory manuals Mycobacterium tuberculosis -- Laboratory manuals Mycobacterium tuberculosis. Microbial genetics. Mycobacterium tuberculosis Tuberculosis -- genetics Genetics, Microbial SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. Microbial genetics Bacterial genetics Mycobacterium tuberculosis Mycobacterium tuberculosis. Tuberculose. Molekulare Medizin Tuberkelbakterium Wetenschappelijke technieken. Tuberculose -- Aspect moléculaire. Mycobacterium tuberculosis. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Parish, Tanya Stoker, Neil G ISBN 9781592591473 0896037762 9780896037762 1592591477 1280821124 9781280821127 9786610821129 6610821127

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