| Description |
xiv, 472 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; 101 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 101
|
| Contents |
Machine derived contents note: Mycobacteria: Bugs and Bugbears -- Tanya Parish and Neil G. Stoker -- Mycobacteria: General Culture Methodology and Safety Considerations -- Bryan W. Allen -- Isolation of Genomic DNA from Mycobacteria -- John T. Belisle and Michael G. Sonnenberg -- Nucleic-Acid Extraction: Plasmid/Cosmid DNA -- Bonnie B. Plikaytis and C. Harold King -- Pulsed Field Gel Electrophoresis for Mycobacteria -- Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and Stewart T. Cole -- Extraction of RNA from Mycobacteria -- Eshwar Mahenthiralingam -- Preparation of Cell-Free Extracts from Mycobacteria -- Tanya Parish and Paul R. Wheeler -- Preparation of Cell-Wall Fractions from Mycobacteria -- Gurdyal S. Besra -- Using MycDB on the World-Wide Web -- Staffan Bergh and Stewart T. Cole -- Conjugating DNA into Mycobacteria -- Julie-Ann Gavigan and Carlos Martin -- Electroporation of Mycobacteria -- Tanya Parish and Neil G. Stoker -- Mycobacteriophages -- Gary J. Sarkis and Graham F. Hatfull -- Chemical Mutagenesis of Mycobacteria -- Lucy A. Brooks -- Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System -- Esther Přez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel, and Carlos Martin -- Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement -- Robert N. Husson -- Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker -- Peter Sander and Erik C. Bṯtger -- Gene Replacement in Mycobacterium intracellulare -- Britt-Inger Marklund and Richard W. Stokes -- Gene Replacement in Mycobacterium bovis BCG -- Elizabeth Norman -- The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria -- Stephen Gordon, Gavin Chung, and Peter Andrew -- Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria -- Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, and V. Deretic -- Expression of Genes in Mycobacteria -- Peadar - Gaora -- Secretion of Mammalian Proteins from Mycobacteria -- Peter J. Murray and Richard A. Young -- Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR -- Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan -- Analysis of Mycobacterial Differential Gene Expression by RAP-PCR -- Joseph A. Mangan and Philip D. Butcher -- Mycobacterial Speciation -- Francis A. Drobniewski and Anne H. C. Uttley -- Species Identification of Mycobacteria Using rDNA Sequencing -- Philip Kirschner and Erik C. Bṯtger -- Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction -- Stuart M. Wilson -- Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping -- Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, and Jan D. A. van Embden -- Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis -- Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden -- Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism) -- Amalio Telenti, Nadine Honor,̌ and Stewart T. Cole -- The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria -- Paul F. Riska and William R. Jacobs, Jr -- Index |
| Notes |
Formerly CIP. Uk |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
Bacterial genetics -- Laboratory manuals.
|
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Mycobacteria -- Laboratory manuals.
|
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Mycobacteria.
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Mycobacterium -- genetics.
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DNA -- physiology.
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Molecular Biology -- methods.
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Mycobacterium -- growth & development.
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DNA -- physiology.
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Molecular Biology -- methods.
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Mycobacterium -- genetics.
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Mycobacterium -- growth & development.
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| Author |
Parish, Tanya.
|
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Stoker, Neil G.
|
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Humana Press.
|
| LC no. |
98022177 |
| ISBN |
0896034712 (alk. paper) |
|
