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Title Human fungal pathogen identification methods and protocols / edited by Thomas Lion, Children's Cancer Research Institute (CCRI), St. Anna Kinderkrebsforschung, Vienna, Austria ; LabDia Labordiagnostik GmbH, Vienna, Austria ; Department of Pediatrics, Medical University of Vienna, Vienna, Austria
Published New York : Humana Press : Springer, ©2017

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Description 1 online resource (xi, 453 pages) : color illustrations
Series Methods in molecular biology ; v. 1508
Methods in molecular biology (Clifton, N.J.) ; v. 1508
Contents Current challenges in the diagnosis of fungal infections /! Cornelia Lass-Flörl --! Changing epidemiology of invasive fungal infections /! David A. Enoch, Huina Yang, Sani H. Aliyu, and Christianne Micallef --! Current algorithms in fungal diagnosis in the immunocompromised host /! Thomas Lehrnbecher, Karsten Becker, and Andreas H. Groll --! Commercial molecular tests for fungal diagnosis from a practical point of view /! Michaela Lackner and Cornelia Lass-Flörl --! Systemic antifungal agents : current status and projected future developments /! Seyedmojtaba Seyedmousavi, Haleh Rafati, Macit Ilkit, Ali Tolooe, Mohammad T. Hedayati, and Paul Verweij --! Fungal-grade reagents and materials for molecular analysis /! Michael G. Lorenz, Michael Lustig, and Marina Linow --! Host-derived biomarkers for risk assessment of invasive fungal diseases /! Cristina Cunha, Samuel M. Gonçalves, and Agostinho Carvalho --! Assessment of immune responses to fungal infections : identification and characterization of immune cells in the infected tissue /! Florian Sparber and Salomé LeibundGut-Landmann --! Histopathology /! Renate Kain --! Culture-based techniques /! Birgit Willinger --! Serological approaches /! Barbora Weinbergerova, Iva Kocmanova, Zdenek Racil, and Jiri Mayer --! Isolation of nucleic acids for fungal diagnosis /! P. Lewis White and Rosemary A. Barnes --! Prerequisites for control of contamination in fungal diagnosis /! Stefan Czurda and Thomas Lion --! Broad-spectrum molecular detection of fungal nucleic acids by PCR-based amplification techniques /! Stefan Czurda and Thomas Lion --! Genus- and species-specific PCR detection methods /! Jan Springer and Jürgen Löffler --! Identification of fungal pathogens in tissue samples from patients with proven invasive infection by fluorescence in situ hybridization /! Ilka McCormick Smith and Volker Rickerts --! Nuclear magnetic resonance spectroscopy-based identification of yeast /! Uwe Himmelreich, Tania C. Sorrell, and Heide-Marie Daniel --! T2 magnetic resonance for fungal diagnosis /! Fainareti N. Zervou, Ioannis M. Zacharioudakis, Jaclynn Kurpewski, and Eleftherios Mylonakis --! Fungal species identification by MALDI-ToF mass spectrometry /! Oliver Bader --! Immunological identification of fungal species /! Filomena Nogueira, Fabian Istel, Leonel Pereira, Michael Tscherner, and Karl Kuchler --! Molecular blueprint of a fungus by next-generation sequencing (NGS) /! Christian Grumaz, Philipp Kirstahler, and Kai Sohn --! Microarray technologies in fungal diagnostics /! Steffen Rupp --! Molecular detection of resistance to echinocandins /! Brunella Posteraro, Antonietta Vella, Elena De Carolis, and Maurizio Sanguinetti --! Molecular detection of resistance to Azole components /! Brunella Posteraro, Antonietta Vella, Elena De Carolis, and Maurizio Sanguinetti --! Immune cell-supplemented human skin model for studying fungal infections /! Andreas Kühbacher, Kai Sohn, Anke Burger-Kentischer, and Steffen Rupp
Summary This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The knowledge of host-related predisposing factors and stratified treatment options facilitating timely onset of adequate antifungal therapy are critical for successful clinical management and outcome of invasive fungal disease (IFD), requiring not only rapid diagnosis of a fungal infection and identification of the causative species, but also assessment of pathogen/host factors related to pathogenicity, susceptibility, and response to treatment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Human Fungal Pathogen Identification: Methods and Protocols serves as an ideal reference for researchers investigating the ever-growing worldwide healthcare problems involving fungal infections
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocols, viewed November 18, 2016)
Subject Diagnostic mycology -- Laboratory manuals
Mushrooms.
Fungi.
Fungi
Mycological Typing Techniques -- methods
Mushrooms
Fungi
Diagnostic mycology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Lion, Thomas, editor.
ISBN 9781493965151
1493965158
1493965131
9781493965137